Correlation between circulating cell-free PIK3CA tumor DNA levels and treatment response in patients with PIK3CA-mutated metastatic breast cancer.
Correlation between circulating cell-free PIK3CA tumor DNA levels and treatment response in patients with PIK3CA-mutated metastatic breast cancer.
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DOI：
10.1002/1878-0261.12305
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发表时间：
2018-06
影响因子：
6.6
通讯作者：
Ditzel HJ
中科院分区：
文献类型：
--
作者：
Kodahl AR;Ehmsen S;Pallisgaard N;Jylling AMB;Jensen JD;Laenkholm AV;Knoop AS;Ditzel HJ
Liquid biopsies focusing on the analysis of cell‐free circulating tumor DNA (ctDNA) may have important clinical implications for personalized medicine, including early detection of cancer, therapeutic guidance, and monitoring of recurrence. Mutations in the oncogene, PIK3CA, are frequently observed in breast cancer and have been suggested as a predictive biomarker for PI3K‐selective inhibitor treatment. In this study, we analyzed the presence of PIK3CA mutations in formalin‐fixed, paraffin‐embedded, metastatic tissue and corresponding ctDNA from serum of patients with advanced breast cancer using a highly sensitive, optimized droplet digital PCR (ddPCR) assay. We found 83% of patients with PIK3CA mutation in the metastatic tumor tissue also had detectable PIK3CA mutations in serum ctDNA. Patients lacking the PIK3CA mutation in corresponding serum ctDNA all had nonvisceral metastatic disease. Four patients with detectable PIK3CA‐mutated ctDNA were followed with an additional serum sample during oncological treatment. In all cases, changes in PIK3CA ctDNA level correlated with treatment response. Our results showed high concordance between detection of PIK3CA mutations in tumor tissue and in corresponding serum ctDNA and suggest that serum samples from patients with advanced breast cancer and ddPCR may be used for PIK3CA mutation status assessment to complement imaging techniques as an early marker of treatment response.
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影响因子：
8.4
作者：
Eisenhauer, E. A.;Therasse, P.;Verweij, J.
通讯作者：
Verweij, J.
影响因子：
3.8
作者：
García-Saenz JA;Ayllón P;Laig M;Acosta-Eyzaguirre D;García-Esquinas M;Montes M;Sanz J;Barquín M;Moreno F;Garcia-Barberan V;Díaz-Rubio E;Caldes T;Romero A
通讯作者：
Romero A
DOI：
10.1073/pnas.0507904102
发表时间：
2005-11-08
影响因子：
11.1
作者：
Diehl, F;Li, M;Vogelstein, B
通讯作者：
Vogelstein, B
影响因子：
7.3
作者：
Buttitta, F;Felicioni, L;Marchetti, A
通讯作者：
Marchetti, A
人类癌症中PIK3CA癌基因的突变。
影响因子：
8.8
作者：
Karakas, B;Bachman, KE;Park, BH
通讯作者：
Park, BH