Quantifying neurotransmitter secretion at single-vesicle resolution using high-density complementary metal-oxide-semiconductor electrode array.
Quantifying neurotransmitter secretion at single-vesicle resolution using high-density complementary metal-oxide-semiconductor electrode array.
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DOI：
10.1038/s41467-020-20267-0
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发表时间：
2021-01-18
影响因子：
16.6
通讯作者：
Kim BN
中科院分区：
文献类型：
--
作者：
White KA;Kim BN
神经元胞吐促进了与身体功能、记忆和情绪有关的信息通过神经系统的传播。利用电流计，可以监测胞吐的亚毫秒动态，并可以研究药物治疗或神经退行性疾病对胞吐的调节。传统的单细胞安培法是研究胞吐作用分子机制的一种有效技术，但要积累具有统计意义的数据既昂贵又费力。为了克服这些限制，我们开发了一种硅基电极阵列，该阵列具有1024个片上电极，可以在0.1毫秒的间隔内测量氧化信号。使用开发的设备，我们能够在记录的30分钟内捕获由于帕金森病治疗（左旋多巴）引起的胞吐调节，具有统计学意义。验证研究证明了我们的设备能够将影响神经递质分泌的各种神经退行性疾病的许多药物治疗的研究加速到几分钟。在单囊泡分辨率下高通量监测神经递质释放仍然是一个挑战。在这里，作者提出了一个硅基电极阵列，包含1024个片上电极，以0.1毫秒的间隔测量神经化学信号，以在记录的30分钟内捕获帕金森病治疗引起的胞吐调节。
Neuronal exocytosis facilitates the propagation of information through the nervous system pertaining to bodily function, memory, and emotions. Using amperometry, the sub-millisecond dynamics of exocytosis can be monitored and the modulation of exocytosis due to drug treatment or neurodegenerative diseases can be studied. Traditional single-cell amperometry is a powerful technique for studying the molecular mechanisms of exocytosis, but it is both costly and labor-intensive to accumulate statistically significant data. To surmount these limitations, we have developed a silicon-based electrode array with 1024 on-chip electrodes that measures oxidative signal in 0.1 millisecond intervals. Using the developed device, we are able to capture the modulation of exocytosis due to Parkinson’s disease treatment (L-Dopa), with statistical significance, within 30 total minutes of recording. The validation study proves our device’s capability to accelerate the study of many pharmaceutical treatments for various neurodegenerative disorders that affect neurotransmitter secretion to a matter of minutes. High-throughput monitoring of neurotransmitter release at single-vesicle resolution remains a challenge. Here, the authors present a silicon-based electrode array with 1024 on-chip electrodes that measures neurochemical signal in 0.1 millisecond intervals to capture the modulation of exocytosis due to Parkinson’s disease treatment within 30 total minutes of recording.
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影响因子：
14
作者：
Sen, Atanu;Barizuddin, Syed;Hossain, Maruf;Polo-Parada, Luis;Gillis, Kevin D.;Gangopadhyay, Shubhra
通讯作者：
Gangopadhyay, Shubhra
影响因子：
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作者：
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4
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Gillis, Winthrop F.;Lissandrello, Charles A.;Gardner, Timothy J.
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Gardner, Timothy J.
影响因子：
21.3
作者：
Alés, E;Tabares, L;de Toledo, GA
通讯作者：
de Toledo, GA
影响因子：
7.4
作者：
Berberian, Khajak;Kisler, Kassandra;Lindau, Manfred
通讯作者：
Lindau, Manfred