Preferential cell attachment to nitrogen-doped diamond-like carbon (DLC:N) for the measurement of quantal exocytosis.
Preferential cell attachment to nitrogen-doped diamond-like carbon (DLC:N) for the measurement of quantal exocytosis.
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DOI：
10.1016/j.biomaterials.2008.11.039
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发表时间：
2009-03
期刊：
影响因子：
14
通讯作者：
Gangopadhyay, Shubhra
中科院分区：
文献类型：
--
作者：
Sen, Atanu;Barizuddin, Syed;Hossain, Maruf;Polo-Parada, Luis;Gillis, Kevin D.;Gangopadhyay, Shubhra
在微芯片上电化学测量单个细胞的递质或激素释放，在基础科学和药物筛选中都有应用。量子胞吐作用的高分辨率测量要求工作电极很小（细胞大小）并位于细胞附近。我们研究了候选电极材料促进两种激素分泌细胞类型附着的能力，作为靶向细胞的机制，以高精度记录电极。我们发现，相对于其他材料，氮掺杂类金刚石碳（DLC:N）促进了细胞的附着，其排列顺序为：DLC:N > In2O3/SnO2 (ITO), Pt > Au。此外，我们发现用聚赖氨酸处理候选电极材料并没有增加染色质细胞对DLC:N的附着，但促进了细胞对其他测试材料的附着。我们发现激素分泌细胞不容易附着在作为潜在绝缘材料的Teflon AF上，并证明Teflon AF的模式导致细胞选择性靶向DLC:N“对接位点”。这些结果将指导下一代生物芯片的设计，用于量子胞吐的自动化和高通量测量。
Electrochemical measurement of transmitter or hormone release from individual cells on microchips has applications both in basic science and drug screening. High-resolution measurement of quantal exocytosis requires the working electrode to be small (cell-sized) and located in immediate proximity to the cell. We examined the ability of candidate electrode materials to promote the attachment of two hormone-secreting cell types as a mechanism for targeting cells for to recording electrodes with high precision. We found that nitrogen-doped diamond-like carbon (DLC:N) promoted cell attachment relative to other materials tested in the rank order of DLC:N > In2O3/SnO2 (ITO), Pt > Au. In addition, we found that treating candidate electrode materials with polylysine did not increase attachment of chromaffin cells to DLC:N, but promoted cell attachment to the other tested materials. We found that hormone-secreting cells did not attach readily to Teflon AF as a potential insulating material, and demonstrated that patterning of Teflon AF leads to selective cell targeting to DLC:N “docking sites”. These results will guide the design of the next generation of biochips for automated and high-throughput measurement of quantal exocytosis.
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