Deconstructing a conserved protein family: the role of MCM proteins in eukaryotic DNA replication.
Deconstructing a conserved protein family: the role of MCM proteins in eukaryotic DNA replication.
复制标题
解构保守蛋白家族：MCM 蛋白在真核 DNA 复制中的作用。
DOI：
10.1007/0-306-47572-3_8
复制
发表时间：
2001
期刊：
影响因子：
--
通讯作者：
S. Forsburg
中科院分区：
文献类型：
--
作者：
S. Pasion;S. Forsburg
细胞分裂是一个精心安排的过程，导致细胞结构、细胞器和遗传物质的复制，然后分裂成两个子细胞。正确的染色体复制和分离是成功完成分裂的必要条件。真核细胞面临着一个特殊的挑战，因为它们相对较大的基因组分布在多个线性染色体中。它们通过协调整个基因组中从复制起点开始的DNA复制来调节S期。为了确保S期在每个细胞周期中只发生一次，在细胞分裂发生和细胞进入下一个周期之前，这些起始点的再起始被阻止。近十年来，基于酵母和裂糖酵母的遗传分析、SV40 DNA复制和非洲爪蟾无细胞系统体外复制的生物化学以及培养细胞的分子研究等互补策略，真核生物DNA复制起始的研究取得了很大进展。在多个系统中的工作提供了一个模型，说明如何在单个原点启动复制（图1）。在这个两步模型中，六聚体起源识别复合体（ORC）在酵母细胞的整个细胞周期中明显地与起源序列结合。第一步是由复制前复合体的组装来定义的，操作上定义为由ORC和复制起始蛋白结合的起点，并且能够开始复制。
Cell division is an exquisitely orchestrated process resulting in the duplication of cellular structures, organelles, and genetic material, followed by its separation into two daughter cells. Proper chromosome replication and segregation are essential for successful completion of division. Eukaryotic cells face a particular challenge with their relatively large genomes divided among multiple linear chromosomes. They regulate S phase by coordinating the initiation of DNA replication from replication origins throughout the genome. To ensure that S phase occurs just once per cell cycle, re-initiation from these origins is prevented until cell division occurs and the cells enter the subsequent cycle. Much progress has been made in dissecting the initiation of DNA replication in eukaryotes over the last ten years, based on complementary strategies including the genetic analysis of the yeasts Saccharomyces cerevisiae and Schizosaccharomyces pombe, the biochemistry of in vitro replication with SV40 DNA replication and Xenopus laevis cell-free systems, and molecular studies in cultured cells. Work in multiple systems has provided a model for how replication is initiated at individual origins (Figure 1). In this two-step model, the hexameric origin recognition complex (ORC) binds the origin sequence apparently throughout the cell cycle in yeast cells. The first step is defined by the assembly of the pre-replicative complex operationally defined as the origin that is bound by ORC and replication initiation proteins and is competent for the initiation of replication.
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裂殖酵母 Cdc19p (MCM2) 的多个结构域需要与核心 MCM 复合物结合。
影响因子：
3.3
作者：
Sherman,DA;Pasion,SG;Forsburg,SL
通讯作者：
Forsburg,SL
影响因子：
7
作者：
A. F. Neuwald;L. Aravind;J. Spouge;E. Koonin
通讯作者：
A. F. Neuwald;L. Aravind;J. Spouge;E. Koonin
使用 cs 和 ts 突变体确定酵母核分裂途径中基因功能的顺序。
影响因子：
3.3
作者：
Moir,D;Botstein,D
通讯作者：
Botstein,D
DOI：
10.1073/pnas.030539597
发表时间：
2000-02-15
影响因子：
11.1
作者：
Chong, JPJ;Hayashi, MK;Stillman, B
通讯作者：
Stillman, B
影响因子：
56.9
作者：
ROGERS, S;WELLS, R;RECHSTEINER, M
通讯作者：
RECHSTEINER, M