A robust process for lipase-mediated biodiesel production from microalgae lipid
A robust process for lipase-mediated biodiesel production from microalgae lipid
复制标题
脂肪酶介导的微藻脂质生产生物柴油的稳健工艺
DOI：
10.1039/c6ra07144a
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发表时间：
2016-05
期刊：
影响因子：
3.9
通讯作者：
Du Wei
中科院分区：
文献类型：
--
作者：
Chen Johnny Zhu;Wang Songmei;Zhou Baiyang;Dai Lingmei;Liu Dehua;Du Wei
微藻油脂因其公认的优势而被认为是生产生物柴油的良好原料。为了促进微藻作为生产生物柴油的原料，开发一种合适的方法将脂肪转化为生物柴油具有重要意义。提出了一种利用游离脂肪酶和固定化脂肪酶相结合的方法转化微藻油脂生产生物柴油的新工艺。这两种脂肪酶的组合被证明能有效地将游离脂肪酸转化为生物柴油(FAME)。系统考察了不同因素对固定化脂肪酶催化甲醇降解的影响。结果表明，添加甲醇的策略、分子筛的用量、脂肪酶的用量和温度对FAME的产率有显著影响。在分子筛用量为10%，醇脂摩尔比为5：1，脂肪酶用量为5%(w/w)，反应温度为45℃，搅拌速度为300rpm的条件下，生物柴油的产率可达97%。在此基础上，采用5水平4因素中心组合设计对固定化脂肪酶介导的醇解反应进行了优化，并系统分析了各变量之间的关系及其对生物柴油产率的共同影响。
Microalgae lipid has been considered a good feedstock for biodiesel production because of its well-recognized advantages. To promote microalgae as a feedstock for biodiesel production, developing an appropriate method to convert the lipid into biodiesel is significant. A novel process with the combination of free lipase and immobilized lipase used for the conversion of microalgae lipid for biodiesel production is proposed in this paper. The combination of the two lipases was demonstrated to be effective in converting free fatty acids into biodiesel (FAME). The effect of different factors influencing immobilized lipase-catalyzed methanolysis was investigated systematically. It was demonstrated that the strategy of adding methanol, molecular sieve dosage, lipase dosage and temperature had significant influence on the FAME yield. Under the optimized conditions (10% 3 A molecular sieve dosage (w/w), molar ratio of methanol to lipid 5:1, lipase dosage 5% (w/w), temperature 45 °C and agitation rate 300 rpm), a biodiesel yield of 97% could be obtained. Further, a 5-level-4-factor central composite design was employed to optimize immobilized lipase-mediated alcoholysis and the relationships between the variables and their co-influence on biodiesel yield were further analyzed systematically.
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影响因子：
11.4
作者：
Alberto Steriti;R. Rossi;A. Concas;G. Cao
通讯作者：
Alberto Steriti;R. Rossi;A. Concas;G. Cao
DOI：
10.1007/s11746-012-2146-3
发表时间：
2013
期刊：
Journal of the American Oil Chemists' Society
影响因子：
--
作者：
H. Ren;Yang Li;Wei Du;De‐hua Liu
通讯作者：
H. Ren;Yang Li;Wei Du;De‐hua Liu
影响因子：
3.9
作者：
Yang Li;Wei Du;De‐hua Liu
通讯作者：
Yang Li;Wei Du;De‐hua Liu
影响因子：
11.2
作者：
Rawat, I.;Kumar, R. Ranjith;Bux, F.
通讯作者：
Bux, F.
影响因子：
1.9
作者：
Griffiths, M. J.;van Hille, R. P.;Harrison, S. T. L.
通讯作者：
Harrison, S. T. L.