LIM only 4 is overexpressed in late stage pancreas cancer.
LIM only 4 is overexpressed in late stage pancreas cancer.
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DOI：
10.1186/1476-4598-7-93
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发表时间：
2008-12-22
期刊：
影响因子：
37.3
通讯作者：
Tanaka M
中科院分区：
文献类型：
--
作者：
Yu J;Ohuchida K;Nakata K;Mizumoto K;Cui L;Fujita H;Yamaguchi H;Egami T;Kitada H;Tanaka M
LIM-Only 4(LMO4)是LIM结构域转录因子中LIM-Only(LMO)亚家族的成员，最初被报道在乳腺癌中起致癌作用。我们推测LMO4可能与胰腺癌的发生有关，就像它在乳腺癌的发生中一样。如果是这样的话，这可能会导致更好地理解胰腺癌的肿瘤发生。我们用实时定量逆转录聚合酶链式反应(qRT-PCR)检测了培养细胞、胰腺实体组织和显微切割的靶细胞(正常导管细胞、胰腺上皮内瘤变-1B[Panin-1B]细胞、Panin-2细胞、浸润性导管癌[IDC]细胞、导管内乳头状粘液性腺瘤[IPMA]细胞、IPM交界区[IPMB]细胞和侵袭性和非侵袭性IPM癌[IPMC])中LMO4的mRNA水平。14个胰腺癌细胞株中有9个细胞株的LMO4基因表达水平高于人胰腺导管上皮细胞系(HPDE)。在大块组织标本中，Lmo4在胰腺癌中的表达高于导管内乳头状黏液性肿瘤或非肿瘤性胰腺组织(p<；0.0001)。我们进行了基于显微解剖的分析。IDC细胞表达Lmo4的水平明显高于正常导管上皮细胞、Panin-1B细胞(P<；0.001)或Panin-2细胞(p=0.014)。IPMC细胞表达Lmo4的水平显著高于正常导管上皮(p<；0.001)、IPMA(p<；0.001)和IPMB细胞(p=0.003)。胰腺癌(IDC和IPMC)中LMO4的表达水平显著高于正常导管上皮、Panin-1B、Panin-2、IPMA和IPMB。这些结果提示，LMO4在胰腺癌的发生发展中处于晚期过表达状态。
LIM-only 4 (LMO4), a member of the LIM-only (LMO) subfamily of LIM domain-containing transcription factors, was initially reported to have an oncogenic role in breast cancer. We hypothesized that LMO4 may be related to pancreatic carcinogenesis as it is in breast carcinogenesis. If so, this could result in a better understanding of tumorigenesis in pancreatic cancer. We measured LMO4 mRNA levels in cultured cells, pancreatic bulk tissues and microdissected target cells (normal ductal cells; pancreatic intraepithelial neoplasia-1B [PanIN-1B] cells; PanIN-2 cells; invasive ductal carcinoma [IDC] cells; intraductal papillary-mucinous adenoma [IPMA] cells; IPM borderline [IPMB] cells; and invasive and non-invasive IPM carcinoma [IPMC]) by quantitative real-time reverse transcription-polymerase chain reaction (qRT-PCR). 9 of 14 pancreatic cancer cell lines expressed higher levels of LMO4 mRNA than did the human pancreatic ductal epithelial cell line (HPDE). In bulk tissue samples, expression of LMO4 was higher in pancreatic carcinoma than in intraductal papillary-mucinous neoplasm (IPMN) or non-neoplastic pancreas (p < 0.0001 for both). We carried out microdissection-based analyses. IDC cells expressed significantly higher levels of LMO4 than did normal ductal epithelia or PanIN-1B cells (p < 0.001 for both) or PanIN-2 cells (p = 0.014). IPMC cells expressed significantly higher levels of LMO4 than did normal ductal epithelia (p < 0.001), IPMA (p < 0.001) and IPMB cells (p = 0.003). Pancreatic carcinomas (both IDC and IPMC) expressed significantly higher levels of LMO4 mRNA than did normal ductal epithelia, PanIN-1B, PanIN-2, IPMA and IPMB. These results suggested that LMO4 is overexpressed at late stages in carcinogenesis of pancreatic cancer.
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DOI：
10.1159/000161009
发表时间：
2008
期刊：
Pancreatology : official journal of the International Association of Pancreatology (IAP) ... [et al.]
影响因子：
--
作者：
Pongprasobchai S;Pannala R;Smyrk TC;Bamlet W;Pitchumoni S;Ougolkov A;de Andrade M;Petersen GM;Chari ST
通讯作者：
Chari ST
DOI：
10.1073/pnas.88.10.4367
发表时间：
1991-05-01
影响因子：
11.1
作者：
BOEHM, T;FORONI, L;RABBITTS, TH
通讯作者：
RABBITTS, TH
影响因子：
6
作者：
Sato, N;Rosty, C;Goggins, M
通讯作者：
Goggins, M
影响因子：
6
作者：
Liu, N;Furukawa, T;Tsao, MS
通讯作者：
Tsao, MS
影响因子：
6.2
作者：
Adsay, NV;Conlon, KC;Klimstra, DS
通讯作者：
Klimstra, DS