16S rRNA gene-based association study identified microbial taxa associated with pork intramuscular fat content in feces and cecum lumen.
16S rRNA gene-based association study identified microbial taxa associated with pork intramuscular fat content in feces and cecum lumen.
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基于 16S rRNA 基因的关联研究确定了与粪便和盲肠腔中猪肉肌内脂肪含量相关的微生物分类群。
DOI：
10.1186/s12866-017-1055-x
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发表时间：
2017-07-19
期刊：
影响因子：
4.2
通讯作者：
Chen C
中科院分区：
文献类型：
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作者：
Fang S;Xiong X;Su Y;Huang L;Chen C
肌内脂肪（IMF）沉积在肌纤维或肌细胞内，是猪的重要肉质性状。以往的研究观察了日粮营养素和添加剂对改善猪肉IMF的影响。肠道微生物组在宿主代谢和能量收获中起着重要作用。肠道微生物群是否对IMF产生影响尚不清楚。在这项研究中，我们调查了500个样品的微生物群落结构，从猪盲肠和粪便中使用高通量16 S rRNA基因测序。我们发现，微生物组的系统发育组成和潜在的功能能力在两种类型的样品之间存在差异。细菌广泛关联研究在两种类型的样品中鉴定出119个与IMF显著相关的OTU（FDR < 0.1）。与IMF相关的OTU大多属于与多糖降解和氨基酸代谢相关的细菌（如普雷沃氏菌属、密螺旋体属、拟杆菌属和梭菌属）。与碳水化合物、能量和氨基酸代谢、细胞运动和膜转运相关的潜在功能能力与IMF含量显著相关。FishTaco分析表明，与IMF相关的微生物组的潜在功能能力的变化可能是由IMF相关的微生物类群引起的。本研究首先评估了肠道微生物组对猪IMF含量的贡献。结果显示了通过调节肠道微生物群来改善IMF的潜在能力。本文的在线版本（doi：10.1186/s12866-017-1055-x）包含补充材料，可供授权用户使用。
Intramuscular fat (IMF) that deposits among muscle fibers or within muscle cells is an important meat quality trait in pigs. Previous studies observed the effects of dietary nutrients and additives on improving the pork IMF. Gut microbiome plays an important role in host metabolism and energy harvest. Whether gut microbiota exerts effect on IMF remains unknown. In this study, we investigated the microbial community structure of 500 samples from porcine cecum and feces using high-throughput 16S rRNA gene sequencing. We found that phylogenetic composition and potential function capacity of microbiome varied between two types of samples. Bacteria wide association study identified 119 OTUs significantly associated with IMF in the two types of samples (FDR < 0.1). Most of the IMF-associated OTUs belong to the bacteria related to polysaccharide degradation and amino acid metabolism (such as Prevotella, Treponema, Bacteroides and Clostridium). Potential function capacities related to metabolisms of carbohydrate, energy and amino acids, cell motility, and membrane transport were significantly associated with IMF content. FishTaco analysis suggested that the shifts of potential function capacities of microbiome associated with IMF might be caused by the IMF-associated microbial taxa. This study firstly evaluated the contribution of gut microbiome to porcine IMF content. The results presented a potential capacity for improving IMF through modulating gut microbiota. The online version of this article (doi:10.1186/s12866-017-1055-x) contains supplementary material, which is available to authorized users.
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影响因子：
7.1
作者：
Cordero, G.;Isabel, B.;Lopez-Bote, C. J.
通讯作者：
Lopez-Bote, C. J.
影响因子：
4.5
作者：
An HM;Park SY;Lee DK;Kim JR;Cha MK;Lee SW;Lim HT;Kim KJ;Ha NJ
通讯作者：
Ha NJ
影响因子：
0.4
作者：
Liang, Y.;Yang, X. M.;Lv, X. B.
通讯作者：
Lv, X. B.
DOI：
10.1073/pnas.1116053109
发表时间：
2012-01-10
影响因子：
11.1
作者：
Greenblum, Sharon;Turnbaugh, Peter J.;Borenstein, Elhanan
通讯作者：
Borenstein, Elhanan
影响因子：
46.9
作者：
通讯作者：
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