The expression and metabolic activity of cytochrome P-450 isozymes in control and phenobarbital-induced primary cultures of rat hepatocytes.
The expression and metabolic activity of cytochrome P-450 isozymes in control and phenobarbital-induced primary cultures of rat hepatocytes.
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对照和苯巴比妥诱导的大鼠肝细胞原代培养物中细胞色素 P-450 同工酶的表达和代谢活性。
DOI：
10.1016/0003-9861(88)90629-7
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发表时间：
1988
影响因子：
3.9
通讯作者：
Pitot,HC
中科院分区：
文献类型：
--
作者：
Turner,NA;Wilson,NM;Jefcoate,CR;Pitot,HC
在T1、Leibovitz L-15 （L-15）和改良的Waymouth 752 （Way）培养基中，研究了苯巴比妥（PB）诱导的P-450同工酶P-450b和P-450e以及主要的3-甲基胆蒽（MC）诱导形式P-450c的表达和活性。通过Western blot分析，对最初分离的肝细胞、对照和pb处理的培养物中的P-450同工酶进行定量分析，并以7,12 -二甲基苯[a]蒽（DMBA）为底物测定其活性。Western blot分析的数据与DMBA的代谢活性密切相关。P-450b在T1和Way的肝细胞中均被PB诱导。P-450e蛋白在最初分离的细胞中构成，在对照培养物中表达水平降低，在所有三种培养基中都通过PB增加或维持。在T1和Way中，PB诱导肝细胞形成与P-450b和P-450e活性相关的DMBA代谢物，并被P-450b抗体抑制。P-450c仅在新鲜制备的肝细胞中很少表达，但在所有对照和pb处理的培养物中都检测到，尽管在T1中表达的水平要高得多。因此，P-450同工酶的数量、PB对它们的诱导作用以及它们对DMBA的活性都与培养基有关。我们已经证明了T1肝细胞中主要的pb诱导同工酶的酶诱导和活性增加；这些也与P-450c表达控制的改变有关，从而增强了苯巴比妥的组成表达和诱导性。
The expression and activity of the phenobarbital (PB)-inducible P-450 isozymes, P-450b and P-450e, and the major 3-methylcholanthrene (MC)-inducible form, P-450c, were studied in primary cultures of adult rat hepatocytes in T1, Leibovitz L-15 (L-15), and a modification of Waymouth 752 1 (Way) media. P-450 isozymes in initially isolated hepatocytes and control and PB-treated cultures were quantitated by Western blot analysis, and activity was determined with 7, 12-dimethylbenz [a] anthracene (DMBA) as substrate. Data from the Western blot analysis correlated well with the metabolic activity toward DMBA. P-450b was consistently induced by PB in hepatocytes in T1 and to a lesser extent in Way. P-450e protein was constitutive in initially isolated cells, expressed in control cultures at a reduced level, and increased or maintained by PB in all three media. DMBA metabolite formation associated with P-450b and P-450e activity was induced by PB in hepatocytes in T1 and Way and was inhibited by antibodies to P-450b. P-450c was only infrequently expressed in freshly prepared hepatocytes, but was detected in all control and PB-treated cultures although at a much higher level in T1. Thus, the amounts of P-450 isozymes, their inducibility by PB, and their activity toward DMBA were found to be dependent on the medium. We have demonstrated enzyme induction and increased activity of the major PB-inducible isozymes in hepatocytes in T1; these are also associated with a change in the control of P-450c expression leading to enhanced constitutive expression and inducibility by phenobarbital.
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哺乳动物肝源性细胞培养物中芳基烃羟化酶的诱导。
影响因子：
3.6
作者：
I. Owens;D. Nebert
通讯作者：
D. Nebert
大鼠肝细胞培养物中细胞色素 P-450 的长期维持和诱导。
影响因子：
5.8
作者：
A. Paine;L. J. Hockin;C. Allen
通讯作者：
C. Allen
与细胞色素 P-450b 和 P-450e 同源的大鼠 mRNA 的组织特异性表达。
影响因子：
14.9
作者：
Omiecinski,CJ
通讯作者：
Omiecinski,CJ
胶原膜上肝实质细胞原代培养物中微粒体细胞色素 b5 和 P-450 的维持。
DOI：
--
发表时间：
1976
期刊：
Life Science
影响因子：
--
作者：
George K. Michalopoulos;G. Sattler;Henry C. Pitot
通讯作者：
Henry C. Pitot
大鼠肝核膜苯并(a)芘代谢和细胞色素P-450异质性特征及与微粒体膜的比较。
影响因子：
4.8
作者：
W. Fahl;C. Jefcoate;C. Kasper
通讯作者：
C. Kasper