The effect of platelet-rich plasma formulations and blood products on human synoviocytes: implications for intra-articular injury and therapy.
The effect of platelet-rich plasma formulations and blood products on human synoviocytes: implications for intra-articular injury and therapy.
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DOI：
10.1177/0363546514525593
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发表时间：
2014-05
期刊：
影响因子：
--
通讯作者：
Dragoo JL
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文献类型：
--
作者：
Braun HJ;Kim HJ;Chu CR;Dragoo JL
富血小板血浆 (PRP) 对软骨细胞的影响已在细胞和组织培养中进行了研究，但对 PRP 对滑膜细胞的影响的关注却少得多。成纤维细胞样滑膜细胞 (FLS) 占正常人类滑膜的 80%，产生可介导软骨分解代谢的细胞因子和基质金属蛋白酶。比较富含白细胞的 PRP (LR-PRP)、缺乏白细胞的 PRP (LP-PRP)、浓缩红细胞 (RBC) 和缺乏血小板的血浆 (PPP) 对人 FLS 的影响，以确定 PRP 配方中的白细胞和红细胞浓度是否对炎症介质的产生产生不同的影响。受控实验室研究。从 4 名捐献者处获取外周血，并对其进行处理以产生 LR-PRP、LP-PRP、RBC 和 PPP。使用标准实验室条件在各自的实验条件下培养人滑膜细胞96小时。然后评估细胞活力和炎症介质的产生。与 LP-PRP (0.72% ± 0.70%; P = .035)、磷酸盐缓冲盐水 (PBS) (0.39% ± 0.27%; P = .018) 和 PPP (0.26% ± 0.30%; P = .013) 相比，LR-PRP 治疗导致滑膜细胞死亡显着增加 (4.9% ± 3.1%)。与 PBS (P < .001)、PPP (P < .001)、LP-PRP (P < .001) 和 LR-PRP (4.9% ± 3.1%; P < .001) 相比，用 RBC 浓缩物处理的滑膜细胞显示出明显更高的细胞死亡 (12.5% ± 6.9%)。与用 PBS 处理的培养物 (0.22 ± 0.295 pg/mL; P < .001)、PPP (0.11 ± 0.179 pg/mL; P < .001) 和红细胞 (0.64 ± 0.58) 处理的培养物相比，用 LR-PRP 处理的培养物中白细胞介素 (IL)–1β 含量显着更高 (1.53 ± 0.86 pg/mL)皮克/毫升；P = .001)。所有其他组中 LR-PRP (32,097.82 ± 22,844.300 pg/mL) 治疗后的 IL-6 含量也较高 (P <.001)。 LP-PRP 中的肿瘤坏死因子-α 水平最高 (9.97 ± 3.110 pg/mL)，并且与所有其他培养条件相比显着更高 (P < .001)。红细胞中的干扰素-γ 水平最高 (64.34 ± 22.987 pg/mL)，并且显着高于所有其他培养条件 (P <.001)。用 LR-PRP 和红细胞处理滑膜细胞导致显着的细胞死亡和促炎介质的产生。关节内给药时，临床医生应考虑使用缺乏白细胞、不含红细胞的 PRP 制剂。
The effect of platelet-rich plasma (PRP) on chondrocytes has been studied in cell and tissue culture, but considerably less attention has been given to the effect of PRP on synoviocytes. Fibroblast-like synoviocytes (FLS) compose 80% of the normal human synovium and produce cytokines and matrix metalloproteinases that can mediate cartilage catabolism. To compare the effects of leukocyte-rich PRP (LR-PRP), leukocyte-poor PRP (LP-PRP), red blood cell (RBC) concentrate, and platelet-poor plasma (PPP) on human FLS to determine whether leukocyte and erythrocyte concentrations of PRP formulations differentially affect the production of inflammatory mediators. Controlled laboratory study. Peripheral blood was obtained from 4 donors and processed to create LR-PRP, LP-PRP, RBCs, and PPP. Human synoviocytes were cultured for 96 hours with the respective experimental conditions using standard laboratory conditions. Cell viability and inflammatory mediator production were then evaluated. Treatment with LR-PRP resulted in significantly greater synoviocyte death (4.9% ± 3.1%) compared with LP-PRP (0.72% ± 0.70%; P = .035), phosphate-buffered saline (PBS) (0.39% ± 0.27%; P = .018), and PPP (0.26% ± 0.30%; P = .013). Synoviocytes treated with RBC concentrate demonstrated significantly greater cell death (12.5% ± 6.9%) compared with PBS (P < .001), PPP (P < .001), LP-PRP (P < .001), and LR-PRP (4.9% ± 3.1%; P < .001). Interleukin (IL)–1β content was significantly higher in cultures treated with LR-PRP (1.53 ± 0.86 pg/mL) compared with those treated with PBS (0.22 ± 0.295 pg/mL; P < .001), PPP (0.11 ± 0.179 pg/mL; P < .001), and RBCs (0.64 ± 0.58 pg/mL; P = .001). IL-6 content was also higher with LR-PRP (32,097.82 ± 22,844.300 pg/mL) treatment in all other groups (P <.001). Tumor necrosis factor–α levels were greatest in LP-PRP (9.97 ± 3.110 pg/mL), and this was significantly greater compared with all other culture conditions (P < .001). Interferon-γ levels were greatest in RBCs (64.34 ± 22.987 pg/mL) and significantly greater than all other culture conditions (P <.001). Treatment of synovial cells with LR-PRP and RBCs resulted in significant cell death and proinflammatory mediator production. Clinicians should consider using leukocyte-poor, RBC-free formulations of PRP when administering intra-articularly.
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DOI：
10.1084/jem.191.2.313
发表时间：
2000-01-17
期刊：
The Journal of experimental medicine
影响因子：
--
作者：
Horai R;Saijo S;Tanioka H;Nakae S;Sudo K;Okahara A;Ikuse T;Asano M;Iwakura Y
通讯作者：
Iwakura Y
影响因子：
27.4
作者：
MORRIS, CJ;BLAKE, DR;STEVEN, MM
通讯作者：
STEVEN, MM
影响因子：
11.4
作者：
KEFFER, J;PROBERT, L;KOLLIAS, G
通讯作者：
KOLLIAS, G
影响因子：
3.3
作者：
Gobbi, Alberto;Karnatzikos, Georgios;Malchira, Somanna
通讯作者：
Malchira, Somanna
影响因子：
4.8
作者：
Dragoo, Jason L.;Braun, Hillary J.;Arnoczky, Steven P.
通讯作者：
Arnoczky, Steven P.