Adipose-derived stem cell sheets promote meniscus regeneration regardless of whether the defect involves the inner half or the whole width of the anterior half of the medial meniscus in a rabbit model.
Adipose-derived stem cell sheets promote meniscus regeneration regardless of whether the defect involves the inner half or the whole width of the anterior half of the medial meniscus in a rabbit model.
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在兔模型中，无论缺陷是否涉及内侧半月板的内半部或前半部的整个宽度，脂肪干细胞片都能促进半月板再生。
DOI：
10.1016/j.arthro.2022.02.020
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发表时间：
2022
期刊：
影响因子：
--
通讯作者：
Tsuchiya H.
中科院分区：
文献类型：
--
作者：
Asai K.;Nakase J;Yoshioka K;Yoshimizu R;Kimura M;Tsuchiya H.
目的探讨脂肪源性干细胞（ADSC）片在兔半月板缺损模型中的再生作用。方法将42只家兔随机分为两组：双膝内侧半月板前半段全部切除（1组）和内半段切除（2组）。ADSC片被移植到一个膝盖，而在另一个膝盖半月板缺陷不进行治疗（自我控制）。比较4周和12周ADSC片处理和未处理的半月板的组织学评分和编码I型和II型胶原（COL1/2）、sly -box转录因子9 （SOX9）和聚集蛋白（ACAN）的基因表达。ADSC片处理12周的半月板也进行免疫组织化学分析，以评估胶原成分。结果两组治疗侧4、12周组织学评分均显著高于对照组（组1，P= 0.016、0.032；组2，P= 0.030、0.032）。016年,分别)。除col2和sox9在第4周、col2在第12周、col1在第2组在第4周、col1在第2组在第4周外，所有评估的基因在两组治疗侧的表达均显著高于对照组。第1组经ADSC片处理的半月板中COL1含量较多，第2组COL1含量较少，但COL2含量丰富。结论无论是内半月板缺损还是全宽半月板缺损，sadsc片均能促进半月板再生。然而，ADSC薄片处理组织的胶原成分因缺陷部位而异。临床意义：由于半月板切除术后半月板缺损，adscs可能有助于半月板再生。该研究建议下一步进行长期随访和力学分析。
PurposeTo investigate the regenerative effect of adipose-derived stem cell (ADSC) sheets in two different rabbit models of meniscal defects.MethodsForty-two rabbits were randomly divided into two groups: the whole (Group 1) or the inner half (Group 2) of anterior half of the medial meniscus was removed from both knees. The ADSC sheets were transplanted into one knee, whereas in the other knee the meniscal defect was left untreated (self-control). The histological score and expression of genes encoding collagen type I and II (COL1/2), SRY-box transcription factor 9 (SOX9), and aggrecan (ACAN) were compared between the ADSC sheet-treated and untreated menisci at 4 and 12 weeks. The ADSC sheet-treated menisci at 12 weeks were also analyzed immunohistochemically to assess the collagen component.ResultsThe histological score was significantly higher in the treated side than in the control side at 4 and 12 weeks in both groups (Group 1;P= .016 and .032; Group 2;P= .030 and .016, respectively). All genes evaluated showed significantly higher expression in the treated side than in the control side in both groups, exceptCOL2andSOX9at 4 weeks andCOL2at 12 weeks in Group 1, andCOL1in Group 2 at 4 weeks. The ADSC sheet-treated meniscus in Group 1 contained mostly COL1, whereas the Group 2 had less COL1, but was rich in COL2.ConclusionsADSC sheets can promote meniscal regeneration regardless of whether the defect involves the inner half or whole width of the anterior half of the medial meniscus. However, the collagen component of the ADSC sheet-treated tissue differs depending on the defect site.Clinical RelevanceADSCs may help meniscal regeneration due to meniscal defects after meniscectomy. This study suggests longer-term follow-up and mechanical analysis as next steps.
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影响因子：
4.2
作者：
D. King
通讯作者：
D. King
影响因子：
4
作者：
Caplan, Arnold I.;Dennis, James E.
通讯作者：
Dennis, James E.
DOI：
10.1007/s11626-014-9782-x
发表时间：
2014-10-01
影响因子：
2.1
作者：
de Sa Barretto, Leticia Siqueira;Lessio, Camila;de Almeida, Fernando Goncalves
通讯作者：
de Almeida, Fernando Goncalves
动态静水压从内部和外部区域不同地增强半月板细胞的软骨形成。
影响因子：
2.4
作者：
J. Zellner;M. Mueller;Y. Xin;W. Krutsch;A. Brandl;R. Kujat;M. Nerlich;P. Angele
通讯作者：
P. Angele