Characterization of endometrial receptors for ovine trophoblast protein-1 during the estrous cycle and early pregnancy in sheep.
Characterization of endometrial receptors for ovine trophoblast protein-1 during the estrous cycle and early pregnancy in sheep.
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绵羊发情周期和妊娠早期子宫内膜受体对绵羊滋养层蛋白-1 的表征。
DOI：
--
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发表时间：
1989
影响因子：
3.6
通讯作者：
G. Niswender
中科院分区：
文献类型：
--
作者：
J. Knickerbocker;G. Niswender
采用Scatchard分析方法，研究绵羊发情周期和妊娠早期子宫内膜中绵羊滋养细胞蛋白-1(OTP-1)受体的分布、数量和亲和力。实验一测定了子宫肌角和肌间区子宫内膜膜OTP-1受体的特性。OTP-1的受体浓度和亲和常数在所研究的四个子宫内膜区之间没有差异(p>0.1)，表明OTP-1受体在子宫内膜中的表达是均匀的。实验二测定了动情周期和妊娠早期子宫内膜OTP-1的受体特性、黄体湿重和孕酮含量。OTP-1的受体浓度和亲和力常数在整个发情周期和妊娠早期都有变化(p<0.01)，周期绵羊和妊娠母羊的变化规律不同(p<0.01)。OTP-1受体在发情周期的第4天达到最大值，到第12天逐渐下降(p<0.05)。12天后，周期绵羊子宫内膜OTP-1受体空白量逐渐增加(p<0.05)至16天，而妊娠绵羊子宫内膜中OTP-1受体空白量进一步下降(p<0.05)。周期绵羊和妊娠绵羊子宫内膜受体对OTP-1的亲和力常数在第12天之前相似，从第4天到第12天增加了3倍(p<0.05)。
Scatchard analysis was used to determine the distribution, number, and affinity of unoccupied receptors for ovine trophoblast protein-1 (oTP-1) in endometrium of sheep throughout the estrous cycle and early pregnancy. In Experiment I, oTP-1 receptor characteristics were determined in membrane preparations of caruncular and intercaruncular regions of endometrium collected from uterine horns ipsilateral and contralateral to the ovary bearing the corpus luteum. Receptor concentrations and affinity constants for oTP-1 were not different (p greater than 0.1) between the four endometrial regions examined, suggesting that the expression of receptors for oTP-1 occurs uniformly throughout the endometrium. Endometrial receptor characteristics for oTP-1, luteal wet weights, and progesterone contents were determined throughout the estrous cycle and early pregnancy in Experiment II. Concentration of receptors and affinity constants for oTP-1 varied throughout the estrous cycle and early pregnancy (p less than 0.01), with the pattern of change differing between cyclic and pregnant ewes (p less than 0.01). Numbers of receptors for oTP-1 were maximal on Day 4 of the estrous cycle and declined progressively to Day 12 (p less than 0.05) in both cyclic and pregnant ewes. After Day 12, the quantity of unoccupied receptors for oTP-1 increased (p less than 0.05) gradually to Day 16 in cyclic ewes, but declined (p less than 0.05) further in the endometrium of pregnant ewes. The affinity constants of endometrial receptors for oTP-1 were similar in cyclic and pregnant ewes prior to Day 12, increasing threefold from Days 4 to 12 (p less than 0.05).(ABSTRACT TRUNCATED AT 250 WORDS)
DOI：
10.1002/0471684228.egp06422
发表时间：
1986
期刊：
CRC critical reviews in biochemistry
影响因子：
--
作者：
M. Rubinstein;P. Orchansky
通讯作者：
M. Rubinstein;P. Orchansky
前列腺素与绵羊黄体细胞亚群的相互作用。
DOI：
10.1016/0090-6980(84)90120-5
发表时间：
1984
期刊：
Prostaglandins
影响因子：
--
作者：
Fitz,TA;Mock,EJ;Mayan,MH;Niswender,GD
通讯作者：
Niswender,GD
牛脾，是纯化 I 型干扰素受体的便捷来源。
DOI：
10.1089/jir.1987.7.77
发表时间：
1987
期刊：
Journal of interferon research
影响因子：
--
作者：
Branca,AA
通讯作者：
Branca,AA