Ultra-small Nd(3+)-doped nanoparticles as near-infrared luminescent biolabels of hemin in bacteria.
Ultra-small Nd(3+)-doped nanoparticles as near-infrared luminescent biolabels of hemin in bacteria.
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超小型 Nd(3) 掺杂纳米粒子作为细菌中血红素的近红外发光生物标记。
DOI：
10.1039/c5nr06106g
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发表时间：
2016-01
期刊：
影响因子：
6.7
通讯作者：
Lin, Hongzhen
中科院分区：
文献类型：
--
作者：
Hua, Hongying;Huang, Yanan;Xiao, Qingbo;Lin, Hongzhen
近红外(NIR)发光的掺钕纳米颗粒(NPs)在生物成像和生物检测方面引起了人们的广泛关注。在这里，我们展示了亚6 nm的NaGdF4：Nd(3+)，Fe(3+)纳米粒子作为氯化血红素分子的发光生物标记，作为微生物群落中的外源电子载体。与上转换或下转换NPs的可见光发光严重猝灭相反，掺Nd3+的NPs在紫外区和可见光范围内表现出了避免氯化血红素的光学吸收的优异性能。详细的测试表明，掺杂Nd3+的纳米粒子对微生物群落没有明显的毒性作用，对标记的氯化高铁血红素分子的特性几乎没有影响，从而增强了发酵体系的还原能力。更重要的是，通过监测掺Nd3+纳米粒子的近红外发光，首次揭示了外源电子载体在缺乏还原能力的细菌中的选择性积累。掺Nd3+纳米粒子作为生物标记物在细菌中的应用将为进一步揭示外源电子载体在厌氧消化中的作用提供新的机会。
Near-infrared (NIR) luminescent Nd(3+)-doped nanoparticles (NPs) have attracted considerable attention in bioimaging and biodetection. Here, we demonstrate sub-6 nm NaGdF4:Nd(3+),Fe(3+) NPs as luminescent biolabels of hemin molecules that act as the exogenous electron carriers in microbial communities. Contrary to the severe quenching of the visible luminescence for either upconverting or downconverting NPs, the Nd(3+)-doped NPs show superior properties in avoiding the optical absorption of hemin within the UV and visible spectral regions. A detailed examination showed that the Nd(3+)-doped NPs exhibit no obvious toxic effects on the microbial communities and show scarce influence on the characteristics of labeled hemin molecules in enhancing the reducing power of the fermentation system. More importantly, by monitoring the NIR luminescence of Nd(3+)-doped NPs, the selective accumulation of exogenous electron carriers in bacteria that are lacking reducing power has been revealed for the first time. The application of Nd(3+)-doped NPs as biolabels in bacteria would provide new opportunities for further unravelling the role of exogenous electron carriers in anaerobic digestion.
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影响因子：
6.7
作者：
Pokhrel M;Mimun LC;Yust B;Kumar GA;Dhanale A;Tang L;Sardar DK
通讯作者：
Sardar DK
DOI：
10.1021/la052035o
发表时间：
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期刊：
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影响因子：
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作者：
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