Spy1 Is Frequently Overexpressed in Malignant Gliomas and Critically Regulates the Proliferation of Glioma Cells
Spy1 Is Frequently Overexpressed in Malignant Gliomas and Critically Regulates the Proliferation of Glioma Cells
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Spy1 在恶性神经胶质瘤中频繁过度表达，并严格调节神经胶质瘤细胞的增殖
DOI：
10.1007/s12031-012-9709-5
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发表时间：
2012-03
影响因子：
3.1
通讯作者：
Cheng, Chun
中科院分区：
文献类型：
--
作者：
Ke, Qing;Zhao, Wei;Yan, Meijuan;Cheng, Chun
Spy1是Speedy/RINGO家族的一员，是周期蛋白依赖性激酶的一种新型激活剂，已知在DNA损伤反应中介导细胞周期进程和细胞存活。本研究的重点是Spy1在胶质瘤肿瘤发生中的作用。免疫组化和western blot检测Spy1在人胶质瘤组织和细胞系中的表达。Spy1在肿瘤组织和培养细胞中频繁过表达。我们的数据表明Spy1的表达与胶质瘤的恶性程度呈正相关。Spy1表达的改变导致细胞周期过程、周期蛋白依赖性激酶2活性和p27kip1蛋白稳定性的改变，最终破坏细胞生长速率。更重要的是，Spy1的高表达与胶质瘤患者预后不良相关，提示Spy1可能是胶质瘤患者生存的一种新的独立预后预测因子。总之，这是首次报道Spy1可能在人类胶质瘤的生长过程中发挥重要作用。
Spy1, a member of the Speedy/RINGO family, is a novel activator of cyclin-dependent kinases known to mediate cell cycle progression and cell survival in response to DNA damage. This study focused on the role of Spy1 in glioma oncogenesis. Immunohistochemistry and western blot analysis were performed to examine the expression of Spy1 in human glioma tissues and cell lines. Spy1 was frequently overexpressed in tumor tissues and cultured cells. Our data suggested that Spy1 expression positively correlated with the malignancy of gliomas. Altered expression of Spy1 led to changes in cell cycle processes, cyclin-dependent kinase 2 activity, and p27kip1 protein stabilization, ultimately disrupting cell growth rate. More importantly, high expression of Spy1 was associated with poor prognosis in patients with glioma, suggesting that Spy1 may be a novel independent prognostic predictor of survival for glioma patients. Collectively, this is the first report that Spy1 may play an essential role in the growth processes of human glioma.
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影响因子：
11.2
作者：
E. Barnes;L. Porter;J. Lenormand;R. Dellinger;D. Donoghue
通讯作者：
E. Barnes;L. Porter;J. Lenormand;R. Dellinger;D. Donoghue
影响因子：
11.2
作者：
Lene Uhrbom;M. Kastemar;Fredrik K Johansson;B. Westermark;E. Holland
通讯作者：
Lene Uhrbom;M. Kastemar;Fredrik K Johansson;B. Westermark;E. Holland
影响因子：
7.5
作者：
Morgan, DO
通讯作者：
Morgan, DO
影响因子：
3.3
作者：
Porter, LA;Kong-Beltran, M;Donoghue, DJ
通讯作者：
Donoghue, DJ
影响因子：
4.3
作者：
McAndrew, Christopher W.;Gastwirt, Randy F.;Donoghue, Daniel J.
通讯作者：
Donoghue, Daniel J.