What lies beneath? Fungal diversity at the bottom of Lake Michigan and Lake Superior.
What lies beneath? Fungal diversity at the bottom of Lake Michigan and Lake Superior.
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DOI：
10.1016/j.jglr.2018.01.001
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发表时间：
2018-04
影响因子：
2.2
通讯作者：
Miller AN
中科院分区：
文献类型：
--
作者：
Wahl HE;Raudabaugh DB;Bach EM;Bone TS;Luttenton MR;Cichewicz RH;Miller AN
真菌是在几乎所有环境中发现的具有系统发育多样性的生物，是养分循环和分解过程的关键贡献者。迄今为止，大多数真菌多样性已经从陆地栖息地记录下来，而水生栖息地尚未得到充分探索。特别是，相对而言，人们对栖息在淡水湖中的真菌知之甚少，特别是底栖区，这可能是真菌生物多样性尚未开发的资源。技术的进步允许从环境样本中直接测序DNA，为研究淡水底栖真菌提供了新的机会。在这项研究中，我们采用了依赖培养和不依赖培养的方法来评估地球上最大的淡水系统之一——北美劳伦森大湖的真菌多样性。本研究首次对来自密歇根湖和苏必利尔湖沉积物的真菌进行了全面调查，结果发现465个真菌分类群，两种方法之间只有7%的序列重叠。此外，ITS1和ITS2区域的培养独立分析显示，分别有49%和72%的otu与真菌界下描述的真菌分类类群不匹配。方法之间的低水平序列重叠和只能在王国水平分类的真菌分类群的高比例表明，在这些水生真菌群落中仍有大量的真菌多样性有待研究。
Fungi are phylogenetically diverse organisms found in nearly every environment as key contributors to the processes of nutrient cycling and decomposition. To date, most fungal diversity has been documented from terrestrial habitats leaving aquatic habitats underexplored. In particular, comparatively little is known about fungi inhabiting freshwater lakes, particularly the benthic zone, which may serve as an untapped resource for fungal biodiversity. Advances in technology allowing for direct sequencing of DNA from environmental samples provide a new opportunity to investigate freshwater benthic fungi. In this study, we employed both culture-dependent and culture-independent methods to evaluate the diversity of fungi in one of the largest freshwater systems on Earth, the North American Laurentian Great Lakes. This study presents the first comprehensive survey of fungi from sediment from Lake Michigan and Lake Superior, resulting in 465 fungal taxa with only 7% of sequence overlap between these two methods. Additionally, culture-independent analyses of the ITS1 and ITS2 regions revealed 49% and 72%, respectively, of the OTUs did not match a described fungal taxonomic group below kingdom Fungi. The low level of sequence overlap between methods and high percentage of fungal taxa that can only be classified at the kingdom level suggests an immense amount of fungal diversity remains to be studied in these aquatic fungal communities.
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影响因子：
5.1
作者：
Edgcomb, Virginia P.;Beaudoin, David;Teske, Andreas
通讯作者：
Teske, Andreas
影响因子：
2.2
作者：
Kiziewicz, Bozena;Nalepa, Thomas F.
通讯作者：
Nalepa, Thomas F.
影响因子：
3.4
作者：
Mueller, Gregory M.;Schmit, John Paul
通讯作者：
Schmit, John Paul
影响因子：
5.8
作者：
Deng, Wenjie;Nickle, David C.;Mullins, James I.
通讯作者：
Mullins, James I.
影响因子：
3.7
作者：
Orsi W;Biddle JF;Edgcomb V
通讯作者：
Edgcomb V