Brain Tumor Biobank Development for Precision Medicine: Role of the Neurosurgeon.
Brain Tumor Biobank Development for Precision Medicine: Role of the Neurosurgeon.
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DOI：
10.3389/fonc.2021.662260
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发表时间：
2021
影响因子：
4.7
通讯作者：
Rodriguez A
中科院分区：
文献类型：
--
作者：
Darrigues E;Elberson BW;De Loose A;Lee MP;Green E;Benton AM;Sink LG;Scott H;Gokden M;Day JD;Rodriguez A
神经肿瘤生物库对于实施精准医学计划至关重要。从这个角度来看，我们回顾了我们第一年的经验，脑肿瘤生物库与集成的下一代测序。根据我们的经验，我们描述了神经外科医生在诊断，研究和精准医学工作中的关键作用。在生物库实施的第一年，117名患者（女性：62名;男性：55名）进行了125次脑肿瘤手术。75%的患者有肿瘤生物库，16%为少数种族/民族。生物库中的肿瘤如下：弥漫性胶质瘤（45%）、脑转移瘤（29%）、脑膜瘤（21%）和其他（5%）。在生物库患者中，100%也进行了下一代测序。11名患者根据可操作基因突变的鉴定有资格接受靶向治疗。还确定了一名患有遗传性癌症易感综合征的患者。实施了迭代质量改进过程，以简化手术室、病理学和研究实验室之间的工作流程。神经外科专门的肿瘤库人员大大提高了标准操作程序。术中选择和处理肿瘤组织的神经外科医生是不可或缺的，以增加成功的细胞培养试验。目前，我们的机构方案整合了标准的组织病理学诊断，下一代测序和手术标本的功能测定，为我们的患者开发精确的医学方案。这一观点回顾了神经外科医生在脑肿瘤生物库实施和成功中的关键作用，以及加强精准医学工作的未来方向。
Neuro-oncology biobanks are critical for the implementation of a precision medicine program. In this perspective, we review our first year experience of a brain tumor biobank with integrated next generation sequencing. From our experience, we describe the critical role of the neurosurgeon in diagnosis, research, and precision medicine efforts. In the first year of implementation of the biobank, 117 patients (Female: 62; Male: 55) had 125 brain tumor surgeries. 75% of patients had tumors biobanked, and 16% were of minority race/ethnicity. Tumors biobanked were as follows: diffuse gliomas (45%), brain metastases (29%), meningioma (21%), and other (5%). Among biobanked patients, 100% also had next generation sequencing. Eleven patients qualified for targeted therapy based on identification of actionable gene mutations. One patient with a hereditary cancer predisposition syndrome was also identified. An iterative quality improvement process was implemented to streamline the workflow between the operating room, pathology, and the research laboratory. Dedicated tumor bank personnel in the department of neurosurgery greatly improved standard operating procedure. Intraoperative selection and processing of tumor tissue by the neurosurgeon was integral to increasing success with cell culture assays. Currently, our institutional protocol integrates standard histopathological diagnosis, next generation sequencing, and functional assays on surgical specimens to develop precision medicine protocols for our patients. This perspective reviews the critical role of neurosurgeons in brain tumor biobank implementation and success as well as future directions for enhancing precision medicine efforts.
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影响因子：
16.6
作者：
Gromeier M;Brown MC;Zhang G;Lin X;Chen Y;Wei Z;Beaubier N;Yan H;He Y;Desjardins A;Herndon JE 2nd;Varn FS;Verhaak RG;Zhao J;Bolognesi DP;Friedman AH;Friedman HS;McSherry F;Muscat AM;Lipp ES;Nair SK;Khasraw M;Peters KB;Randazzo D;Sampson JH;McLendon RE;Bigner DD;Ashley DM
通讯作者：
Ashley DM
影响因子：
4.7
作者：
Han CH;Brastianos PK
通讯作者：
Brastianos PK
影响因子：
15.9
作者：
Dankner, Matthew;Caron, Maxime;Siegel, Peter M.
通讯作者：
Siegel, Peter M.
影响因子：
18.4
作者：
Hambardzumyan D;Bergers G
通讯作者：
Bergers G
影响因子：
16.6
作者：
Harutyunyan, Ashot S.;Krug, Brian;Majewski, Jacek
通讯作者：
Majewski, Jacek