Targeted gene transfection from microbubbles into vascular smooth muscle cells using focused, ultrasound-mediated delivery.
Targeted gene transfection from microbubbles into vascular smooth muscle cells using focused, ultrasound-mediated delivery.
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DOI：
10.1016/j.ultrasmedbio.2010.06.010
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发表时间：
2010-09
影响因子：
2.9
通讯作者：
Hossack, John A.
中科院分区：
文献类型：
--
作者：
Phillips, Linsey C.;Klibanov, Alexander L.;Wamhoff, Brian R.;Hossack, John A.
我们研究了一种利用超声触发的质粒DNA从静电偶联的阳离子微泡中递送到血管平滑肌细胞的基因递送方法。携带报告质粒DNA的微泡在一定范围的声压（0-950 kPa）和脉冲持续时间（0-100个周期）下在体外平滑肌细胞附近声破裂。单独应用微泡、DNA或超声波对基因转染或活力没有观察到任何影响。微泡与超声波（500 kPa，1 MHz，50个周期，脉冲重复频率[PRF]为100 Hz）的组合显着降低了与DNA（53 +/-27%）和无（19 +/-8%）的活力。在300 kPa下使用50个循环、1 MHz脉冲发生最大基因转染（约1%的细胞），导致40%的细胞活力。我们证明，我们可以在体外使用微泡载体和聚焦超声局部递送DNA到血管平滑肌细胞。
We investigate a method for gene delivery to vascular smooth muscle cells using ultrasound triggered delivery of plasmid DNA from electrostatically coupled cationic microbubbles. Microbubbles carrying reporter plasmid DNA were acoustically ruptured in the vicinity of smooth muscle cells in vitro under a range of acoustic pressures (0–950 kPa) and pulse durations (0–100 cycles). No effect on gene transfection or viability was observed from application of microbubbles, DNA, or ultrasound alone. Microbubbles in combination with ultrasound (500 kPa, 1MHz, 50 cycle bursts at a Pulse Repetition Frequency [PRF] of 100 Hz) significantly reduced viability both with DNA (53 +/− 27%) and without (19 +/− 8%). Maximal gene transfection (~1% of cells) occurred using 50 cycle, 1 MHz pulses at 300 kPa which resulted in 40% viability of cells. We demonstrated that we can locally deliver DNA to vascular smooth muscle cells in vitro using microbubble carriers and focused ultrasound.
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影响因子：
2.4
作者：
Guzmán, HR;Nguyen, DX;Prausnitz, MR
通讯作者：
Prausnitz, MR
DOI：
10.1109/58.741536
发表时间：
1999-01-01
影响因子：
3.6
作者：
Dayton, PA;Morgan, KE;Ferrara, KW
通讯作者：
Ferrara, KW
影响因子：
2.9
作者：
Karshafian, Raffi;Bevan, Peter D.;Burns, Peter N.
通讯作者：
Burns, Peter N.
影响因子：
2.9
作者：
Miller, DL;Dou, CY;Song, JM
通讯作者：
Song, JM
影响因子：
1.7
作者：
Phillips, Linsey C.;Klibanov, Alexander L.;Wamhoff, Brian R.
通讯作者：
Wamhoff, Brian R.