Overexpression of an Osa-miR162a Derivative in Rice Confers Cross-Kingdom RNA Interference-Mediated Brown Planthopper Resistance without Perturbing Host Development.
Overexpression of an Osa-miR162a Derivative in Rice Confers Cross-Kingdom RNA Interference-Mediated Brown Planthopper Resistance without Perturbing Host Development.
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Osa-miR 162 a衍生物在水稻中的过表达赋予跨王国RNA干扰介导的褐飞虱抗性而不干扰宿主发育
DOI：
10.3390/ijms222312652
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发表时间：
2021-11-23
影响因子：
5.6
通讯作者：
Li JF
中科院分区：
文献类型：
--
作者：
Shen W;Cao S;Liu J;Zhang W;Chen J;Li JF
水稻是全球一半以上人口的主要粮食作物。褐飞虱（Nilaparvata lugens）是水稻生产上最具破坏性的害虫之一。目前，重复过度使用化学杀虫剂是农业中控制BPH的常见做法，这可以诱导昆虫耐受性并引起环境问题。这种情况需要创新和广泛适用的策略，以保护水稻免受褐飞虱。在这里，我们报告了水稻osa-miR 162 a可以介导跨王国RNA干扰（RNAi）靶向NlTOR（雷帕霉素的目标）基因的BPH，调节生殖过程。通过人工饲料或注射，osa-miR 162 a模拟物抑制了NlTOR的表达并损害了BPH成虫的产卵。因此，在转基因水稻植株中过量产生的osa-miR 162 a损害了以这些植物喂养的BPH成虫的繁殖力，但同时干扰了根和谷粒的发育。为了避免这个问题，我们通过降低与水稻内源靶基因的碱基互补性同时增加与NlTOR的碱基互补性来产生osa-miR 162 a-m1，一种序列优化的osa-miR 162 a。osa-miR 162 a-m1的转基因过表达赋予水稻对BPH的抗性，而没有可检测到的发育惩罚。本研究首次揭示了水稻与褐飞虱相互作用的跨界RNAi机制，为提高水稻对褐飞虱的抗性提供了一种可能的途径。我们还引入了一种有效的策略，将不需要的宿主发育干扰与过表达植物miRNA的跨界RNAi益处分开。
Rice is a main food crop for more than half of the global population. The brown planthopper (BPH, Nilaparvata lugens) is one of the most destructive insect pests of rice. Currently, repeated overuse of chemical insecticides represents a common practice in agriculture for BPH control, which can induce insect tolerance and provoke environmental concerns. This situation calls for innovative and widely applicable strategies for rice protection against BPH. Here we report that the rice osa-miR162a can mediate cross-kingdom RNA interference (RNAi) by targeting the NlTOR (Target of rapamycin) gene of BPH that regulates the reproduction process. Through artificial diet or injection, osa-miR162a mimics repressed the NlTOR expression and impaired the oviposition of BPH adults. Consistently, overproduced osa-miR162a in transgenic rice plants compromised the fecundity of BPH adults fed with these plants, but meanwhile perturbed root and grain development. To circumvent this issue, we generated osa-miR162a-m1, a sequence-optimized osa-miR162a, by decreasing base complementarity to rice endogenous target genes while increasing base complementarity to NlTOR. Transgenic overexpression of osa-miR162a-m1 conferred rice resistance to BPH without detectable developmental penalty. This work reveals the first cross-kingdom RNAi mechanism in rice-BPH interactions and inspires a potentially useful approach for improving rice resistance to BPH. We also introduce an effective strategy to uncouple unwanted host developmental perturbation from desirable cross-kingdom RNAi benefits for overexpressed plant miRNAs.
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影响因子：
3.8
作者：
Ling, Lin;Ge, Xie;Tan, Anjiang
通讯作者：
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10.1073/pnas.0907391106
发表时间：
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影响因子：
11.1
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