Changes in the organization of the surface membrane upon transformation of cercariae to schistosomula of the helminth parasite Schistosoma mansoni
Changes in the organization of the surface membrane upon transformation of cercariae to schistosomula of the helminth parasite Schistosoma mansoni
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曼氏血吸虫尾蚴转化为血吸虫后表面膜组织的变化
DOI：
10.1017/s0031182000081671
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发表时间：
1988
期刊：
影响因子：
2.4
通讯作者：
P. Garland
中科院分区：
文献类型：
--
作者：
M. Foley;J. Kusel;P. Garland
摘要 部花青蛋白 540 (Mc540) 是一种荧光化合物，被认为可以与脂质结晶相中存在大量脂质的膜结合。这里显示它对于通过机械和皮肤穿透方法检测尾蚴向血吸虫的转化具有价值。尾蚴似乎不结合 Mc540，但血吸虫最初在其前部区域结合 Mc540，随后在整个表面结合。转化涉及表面膜脂质相从凝胶到液晶相的变化的建议得到了 5-N-(十八酰基)-氨基荧光素光漂白结果后荧光恢复的支持，5-N-(十八烷酰基)-氨基荧光素是一种亲脂性染料，在尾蚴中似乎是固定的，但在 40 分钟血吸虫中完全可移动。
SUMMARY Merocyanin 540 (Mc540) is a fluorescent compound which is thought to bind to membranes in which there are substantial amounts of lipid in the lipid-crystalline phase. It is shown here to be of value in detecting the transformation by both mechanical and skin-penetration methods of the cercaria to the schistosomulum. The cercaria does not appear to bind Mc540, but the schistosomulum, binds Mc540 initially, in its anterior region, and at later times over the entire surface. The suggestion that transformation involves changes in the surface membrane lipid phase from gel to liquid-crystalline phase is supported by fluorescence recovery after photobleaching results with 5-N-(octadecanoyl)-amino fluorescein, a lipophilic dye which appears to be immobile in the cercaria, but fully mobile in the 40 min schistosomulum.
DOI：
10.1016/0167-4889(84)90009-0
发表时间：
1984
期刊：
Biochimica et biophysica acta
影响因子：
--
作者：
Freidus,DJ;Schlegel,RA;Williamson,P
通讯作者：
Williamson,P