A chimeric virus-based probe unambiguously detects live circulating tumor cells with high specificity and sensitivity.
A chimeric virus-based probe unambiguously detects live circulating tumor cells with high specificity and sensitivity.
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DOI：
10.1016/j.omtm.2021.08.007
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发表时间：
2021-12-10
期刊：
影响因子：
--
通讯作者：
Zhang X
中科院分区：
文献类型：
--
作者：
Fu X;Tao L;Zhang X
目前用于检测循环肿瘤细胞（CTC）的方法具有若干缺点。我们报告了一种基于嵌合病毒探针的新方法，可以以极高的特异性和灵敏度检测CTC。此外，它专门检测活CTC，其检测功效不受上皮细胞粘附分子（EpCAM）表达变化的影响。嵌合病毒探针由来自人乳头瘤病毒的衣壳和基于SV40的基因组组成，所述衣壳提供高特异性的检测，所述基于SV40的基因组可以在CTC内广泛扩增，因此赋予检测高灵敏度。此外，可以将不同的标记基因掺入探针中以提供具有通用性的检测。这些独特的能力将可能提高这种CTC检测在几种临床应用中的有效性和实用性，这是许多当前CTC检测方法所遭受的缺点之一。设计了一种混合病毒探针，其结合了来自人乳头瘤病毒（HPV）和SV40的组分，前者提供上皮特异性感染以使探针高度选择性地进入循环肿瘤细胞（CTC），后者提供扩增以进行灵敏检测。最重要的是，这种检测方法确保所有检测到的CTC都是活的。
The current methods for detecting circulating tumor cells (CTCs) suffer from several drawbacks. We report a novel method that is based on a chimeric virus probe and can detect CTCs with extremely high specificity and sensitivity. Moreover, it exclusively detects live CTCs, and its detection efficacy is not impacted by the variation of epithelial cell adhesion molecule (EpCAM) expression. The chimeric virus probe is composed of a capsid from human papillomavirus that provides the detection with high specificity and an SV40-based genome that can amplify extensively inside CTCs and, hence, endows the detection with high sensitivity. Furthermore, different marker genes can be incorporated into the probe to provide detection with versatility. These unique capabilities will likely improve the validity and utility of this CTC detection in several clinical applications, which is one of the drawbacks suffered by many of the current CTC detection methods. A hybrid virus probe is designed that incorporates components from both human papillomavirus (HPV) and SV40, with the former providing epithelium-specific infection for a highly selective entry of the probe into circulating tumor cells (CTCs) and the latter providing amplification for sensitive detection. Most importantly, such a detection method ensures that all the detected CTCs are alive.
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通讯作者：
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