Shotgun proteomics of plant plasma membrane and microdomain proteins using nano-LC-MS/MS.
Shotgun proteomics of plant plasma membrane and microdomain proteins using nano-LC-MS/MS.
复制标题
使用纳米 LC-MS/MS 对植物质膜和微域蛋白进行鸟枪法蛋白质组学研究。
DOI：
10.1007/978-1-62703-631-3_33
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发表时间：
2014
影响因子：
--
通讯作者：
M. Uemura
中科院分区：
文献类型：
--
作者：
D. Takahashi;Bin Li;Takato Nakayama;Y. Kawamura;M. Uemura
鸟枪蛋白质组学允许对从植物细胞、亚细胞细胞器和膜中提取的蛋白质进行全面分析。以前，基于二维凝胶电泳的蛋白质组学被用于质谱分析质膜蛋白。为了获得包括具有多个跨膜结构域的高度疏水蛋白在内的质膜的全面蛋白质组学图谱，本文描述了一种基于质谱的霰弹枪蛋白质组学方法，该方法使用纳米lc -MS/MS对质膜蛋白和质膜微结构域部分的蛋白质进行分析。所得结果易于应用于无标记蛋白半定量。
Shotgun proteomics allows the comprehensive analysis of proteins extracted from plant cells, subcellular organelles, and membranes. Previously, two-dimensional gel electrophoresis-based proteomics was used for mass spectrometric analysis of plasma membrane proteins. In order to get comprehensive proteome profiles of the plasma membrane including highly hydrophobic proteins with a number of transmembrane domains, a mass spectrometry-based shotgun proteomics method using nano-LC-MS/MS for proteins from the plasma membrane proteins and plasma membrane microdomain fraction is described. The results obtained are easily applicable to label-free protein semiquantification.
影响因子：
4.9
作者：
Li, Bin;Takahashi, Daisuke;Uemura, Matsuo
通讯作者：
Uemura, Matsuo