Band 4.1 proteins regulate integrin-dependent cell spreading.
Band 4.1 proteins regulate integrin-dependent cell spreading.
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DOI：
10.1016/j.bbrc.2012.08.129
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发表时间：
2012-10-05
影响因子：
3.1
通讯作者：
McCarty, Joseph H.
中科院分区：
文献类型：
--
作者：
Jung, Youngsin;McCarty, Joseph H.
Integrins link the extracellular matrix (ECM) to the cytoskeleton to control cell behaviors including adhesion, spreading and migration. Band 4.1 proteins contain 4.1, ezrin, radixin, moesin (FERM) domains that likely mediate signaling events and cytoskeletal reorganization via integrins. However, the mechanisms by which Band 4.1 proteins and integrins are functionally interconnected remain enigmatic. Here we have investigated roles for Band 4.1 proteins in integrin-mediated cell spreading. We demonstrate that Proteins 4.1B and 4.1G show overlapping and dynamic patterns of sub-cellular localization in astrocytes spreading on fibronectin. During early stages of cell spreading Proteins 4.1B and 4.1G are enriched in ECM adhesion sites but become more diffusely expressed in later stages of cell spreading. Combinatorial inhibition of Protein 4.1B and 4.1G expression leads to impaired astrocyte spreading. Furthermore, using exogenous expression systems we show that the isolated Protein 4.1 FERM domain significantly enhances β1 integrin-mediated cell spreading. Lastly, Protein 4.1B is dispensable for reactive astrogliosis in experimental models of cortical injury, likely due to functional compensation by related Protein 4.1 family members. Collectively, these findings reveal that Band 4.1 proteins are important intracellular components for integrin-mediated cell spreading.
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DOI：
10.1523/jneurosci.5225-09.2010
发表时间：
2010-02-17
期刊：
The Journal of neuroscience : the official journal of the Society for Neuroscience
影响因子：
--
作者：
Horresh I;Bar V;Kissil JL;Peles E
通讯作者：
Peles E
影响因子：
4
作者：
Ruiz-Saenz, Ana;Kremer, Leonor;Correas, Isabel
通讯作者：
Correas, Isabel
影响因子：
13.9
作者：
Kriegstein A;Alvarez-Buylla A
通讯作者：
Alvarez-Buylla A
影响因子：
6.2
作者：
Burns, Kevin A.;Murphy, Brian;Kuan, Chia-Yi
通讯作者：
Kuan, Chia-Yi
影响因子：
2.9
作者：
Okumura, Kazuhiro;Mochizuki, Eiji;Kikkawa, Yoshiaki
通讯作者：
Kikkawa, Yoshiaki