Steroidogenic acute regulatory protein transcript abundance in the eel, Anguilla australis: Changes during the induced reproductive cycle and effects of follicle-stimulating hormone during previtellogenesis
Steroidogenic acute regulatory protein transcript abundance in the eel, Anguilla australis: Changes during the induced reproductive cycle and effects of follicle-stimulating hormone during previtellogenesis
复制标题
澳大利亚鳗鲡中类固醇生成急性调节蛋白转录物丰度：诱导生殖周期中的变化以及卵黄发生前促卵泡激素的影响
DOI：
10.1016/j.jsbmb.2013.02.003
复制
发表时间：
2013
期刊：
影响因子：
--
通讯作者：
P. Lokman
中科院分区：
文献类型：
--
作者：
Peter M. Reid;Sean L. Divers;V. Zadmajid;Khalid M. Alqaisi;P. Lokman
采用定量PCR方法测定了鳗鱼卵巢中类固醇生成急性调节蛋白（星星）mRNA水平，并将其与卵子发生过程中血浆类固醇水平进行相关性分析，以阐明先前认为的血浆雌二醇（E2）水平的“异常”早熟性升高。总卵巢星星转录丰度反映了E2的循环水平，但不是11-酮睾酮（11 KT）。该研究通过评价促卵泡激素（FSH）对卵巢星星转录本丰度的体外影响和对卵巢碎片的短期（“急性”）放射性标记的炔雌醇支持的类固醇代谢的体外影响来补充，以了解在pretellogenic卵母细胞生长期间类固醇的产生是如何调节的。我们观察到FSH对星星mRNA水平在孵育24小时内有显著影响，但培养4天后这些影响不再明显。出乎意料的是，FSH对底物支持的类固醇生成没有影响，因为使用半定量HPLC和闪烁计数检测到类固醇产物的产率相当。我们的结论是鳗鱼卵泡可以响应FSH从一个非常早期的发展阶段（早期油滴阶段）通过增加星星mRNA水平，但没有证据表明FSH对细胞色素P450侧链裂解下游生物活性类固醇生产的急性影响。此外，体内星星mRNA的早熟增加与一般硬骨鱼模型一致，可能是达到发育晚期的“正常”反应。
Steroidogenic acute regulatory protein (StAR) mRNA levels in the eel ovary were assayed by quantitative PCR and related to plasma steroid levels throughout oogenesis in order to shed light on the previously considered ‘aberrant’ prematurational increase in plasma levels of estradiol-17β (E2). Total ovarian StAR transcript abundance mirrored circulating levels of E2, but not of 11-ketotestosterone (11KT). The study was complemented by evaluation of in vitro effects of follicle-stimulating hormone (FSH) on ovarian StAR transcript abundance and on short-term (‘acute’) radiolabelled pregnenolone-supported steroid metabolism by ovarian fragments to understand how the production of steroids during previtellogenic oocyte growth is regulated. We observed a significant effect of FSH on StAR mRNA levels within 24 h of incubation, but these were no longer evident by 4 days of culture. Unexpectedly, FSH had no effect on substrate-supported steroidogenesis, as comparable yields of steroid products were detected using semi-quantitative HPLC and scintillation counting. We conclude that the eel ovarian follicle can respond to FSH from a very early stage of development (early oil droplet stage) by increasing StAR mRNA levels, but that there is no evidence for acute effects of FSH on bioactive steroid production downstream of cytochrome P450 side-chain cleavage. Furthermore, the prematurational increase in StAR mRNA in vivo is in keeping with general teleost models and is likely to be a ‘normal’ response to reaching advanced stages of development.
影响因子：
4
作者：
Stocco, DM
通讯作者：
Stocco, DM
影响因子：
56.9
作者：
LIN, D;SUGAWARA, T;MILLER, WL
通讯作者：
MILLER, WL