Segment-specific expression of a homoeobox-containing gene in the mouse hindbrain
Segment-specific expression of a homoeobox-containing gene in the mouse hindbrain
复制标题
小鼠后脑中含有同源异型盒的基因的片段特异性表达
作者：
P. Murphy;D. Davidson;R. Hill
发育中的胚胎沿着其前后轴被分成重复的结构的分割过程，作为组织和协调身体计划的一种手段，在许多生物中都有发现。在果蝇中，同型基因参与了各个层次的节段组织和决定节段身份1。这些基因在片段发育中的作用主要是通过突变研究发现的[2,3]，但也通过原位杂交发现了它们的表达域。然而，与果蝇相反，在脊椎动物生物体中，含有同源盒的基因的胚胎表达并没有被发现遵循一种片段模式。在中胚层体中可以清楚地看到脊椎动物的分割，但中枢神经系统（神经粒）5-13的重复形态结构直到最近才被证明具有发育意义。后脑的神经粒被定义为节段单位，通过它们在发育中的小鸡中的神经形成模式和在9.5天大的小鼠中编码锌指dna结合蛋白的基因Krox-20的交替表达。在这里，我们报道了一个含有小鼠同源盒的基因，Hox-2.9，在发育中的小鼠后脑中以片段特异性的方式表达。这种表达位于Krox-20表达区两侧的区域，并且精确地包含在单个神经细胞菱形体4中。
THE process of segmentation, in which the developing embryo is divided into repetitive structures along its antero–posterior (A–P) axis, as a means of organizing and coordinating the body plan is found in a wide range of organisms. In Drosophila, homoeotic genes are involved in all levels of segmental organization and in determining segment identity1. The roles of these genes in seg-mentation have been found mainly by mutational studies2,3, but also by in situ hybridization, which has shown their domains of expression. In contrast to Drosophila, however, embryonic expression of homoeobox-containing genes in vertebrate organisms has not been found to follow a segmental pattern4. Vertebrate segmentation can be clearly seen in the mesodermal somites, but repetitive morphological structures in the central nervous system (neuromeres)5–13 have only recently been shown to have develop-mental significance. Neuromeres in the hindbrain (rhombomeres) have been defined as segmental units by their pattern of nerve formation in the developing chick14 and by the alternating expression of Krox-20, a gene encoding a zinc-finger DNA-binding protein, in the 9.5-day-old mouse15. Here we report that a mouse homoeobox-containing gene, Hox-2.9, is expressed in a segment-specific manner in the developing mouse hindbrain. This expression is in a region which is flanked by the regions of expression of Krox-20, and is precisely contained within a single neuromere, rhombomere 4.
影响因子：
10.5
作者：
DIEDERICH, RJ;MERRILL, VKL;KAUFMAN, TC
通讯作者：
KAUFMAN, TC
果蝇胚胎头部同源异型蛋白积累的新模式。
DOI：
10.1242/dev.105.1.167
发表时间：
1989
期刊：
Development (Cambridge, England)
影响因子：
--
作者：
Mahaffey,JW;Diederich,RJ;Kaufman,TC
通讯作者：
Kaufman,TC