ADP-ribosylation of the rho/rac proteins induces growth inhibition, neurite outgrowth and acetylcholine esterase in cultured PC-12 cells.
ADP-ribosylation of the rho/rac proteins induces growth inhibition, neurite outgrowth and acetylcholine esterase in cultured PC-12 cells.
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rho/rac 蛋白的 ADP-核糖基化可诱导培养的 PC-12 细胞的生长抑制、神经突生长和乙酰胆碱酯酶。
DOI：
10.1016/0006-291x(90)91760-p
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发表时间：
1990
影响因子：
3.1
通讯作者：
S. Kozaki
中科院分区：
文献类型：
--
作者：
T. Nishiki;S. Narumiya;N. Morii;Masamitsu Yamamoto;M. Fujiwara;Yoichi Kamata;G. Sakaguchi;S. Kozaki
摘要将肉毒杆菌adp -核糖基转移酶C3 （C3外酶）纯化至均匀性，并加入培养的大鼠嗜铬细胞瘤PC-12细胞中。用这种外泌酶孵育引起细胞生长抑制，并在这些细胞中诱导神经突和乙酰胆碱酯酶。这些变化取决于添加到培养物中的酶的量，这与细胞中rho rac蛋白的原位adp核糖基化有关。用特异性抗c3外酶单克隆抗体预孵育可抑制adp -核糖基转移酶活性和酶制剂的神经突诱导活性。这些结果表明，C3外泌酶通过adp -核糖基化作用影响rho rac蛋白的细胞功能，从而引起细胞的这些变化。
Abstract Botulinum ADP-ribosyltransferase C3 (C3 exoenzyme) was purified to homogeneity and added to cultured rat pheochromocytoma PC-12 cells. Incubation with this exoenzyme caused inhibition of cell growth and induced neurites as well as acetylcholine esterase in these cells. These changes were dependent on the amount of the enzyme added to the culture, which correlated with the in situ ADP-ribosylation of the rho rac proteins in the cells. Preincubation with a specific anti-C3 exoenzyme monoclonal antibody inhibited both the ADP-ribosyl-transferase activity and the neurite-inducing activity of the enzyme preparation. These results suggest that C3 exoenzyme affected the cellular function of the rho rac proteins by ADP-ribosylation to induce these changes in the cells.
影响因子：
4.1
作者：
Gibson, RM;Wilson-Delfosse, AL
通讯作者：
Wilson-Delfosse, AL
当肉毒杆菌外切酶 C3 ADP 核糖基化时，21 千道尔顿 G 蛋白的功能修饰。
影响因子：
5.3
作者：
Rubin,EJ;Gill,DM;Boquet,P;Popoff,MR
通讯作者：
Popoff,MR