Validation of novel promoter sequences derived from two endogenous ubiquitin genes in transgenic Aedes aegypti.
Validation of novel promoter sequences derived from two endogenous ubiquitin genes in transgenic Aedes aegypti.
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DOI：
10.1111/j.1365-2583.2010.01005.x
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发表时间：
2010-08
影响因子：
2.6
通讯作者：
Adelman ZN
中科院分区：
文献类型：
--
作者：
Anderson MA;Gross TL;Myles KM;Adelman ZN
迄今为止，仅描述了有限数量的启动子序列来驱动疾病载体埃及伊蚊中的转基因表达。我们试图通过表征源自伊蚊的上游序列的能力来增加这个库。埃及伊蚊 UbL40 和多聚泛素基因驱动标记蛋白的表达。两种基因组片段都能够在培养的蚊子细胞中驱动荧光素酶的强劲表达。 Mos1 转化后，UbL40 启动子在早期幼虫和卵巢中驱动荧光标记的强烈表达，而多聚泛素启动子在发育的所有阶段驱动 EGFP 强烈表达，包括整个中肠的组成型表达。这些启动子片段为未来的 Ae 提供了两种新的表达谱。埃及伊蚊基因实验。
To date, only a limited number of promoter sequences have been described to drive transgene expression in the disease vector Aedes aegypti. We sought to increase this repertoire by characterizing the ability of upstream sequences derived from the Ae. aegypti UbL40 and polyubiquitin genes to drive the expression of marker proteins. Both genomic fragments were able to drive robust expression of luciferase in cultured mosquito cells. Following Mos1-transformation, the UbL40 promoter drove strong expression of a fluorescent marker in early larvae and in ovaries, while the polyubiquitin promoter drove robust EGFP expression in all stages of development including constitutive expression throughout the midgut. These promoter fragments provide two new expression profiles for future Ae. aegypti genetic experiments.
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影响因子：
2.6
作者：
Lorenzen, MD;Brown, SJ;Beeman, RW
通讯作者：
Beeman, RW
影响因子：
3.8
作者：
Antonova, Yevgeniya;Alvarez, Kanwal S.;Kim, Yu Jung;Kokoza, Vladimir;Raikhel, Alexander S.
通讯作者：
Raikhel, Alexander S.
DOI：
10.1073/pnas.0701515104
发表时间：
2007-06-12
影响因子：
11.1
作者：
Adelman, Zach N.;Jasinskiene, Nijole;James, Anthony A.
通讯作者：
James, Anthony A.
影响因子：
2.4
作者：
Horn, C;Wimmer, EA
通讯作者：
Wimmer, EA
影响因子：
3.8
作者：
Adelman, Zach N.;Anderson, Michelle A. E.;Myles, Kevin M.
通讯作者：
Myles, Kevin M.