Clostridium perfringens delta toxin is sequence related to beta toxin, NetB, and Staphylococcus pore-forming toxins, but shows functional differences.
Clostridium perfringens delta toxin is sequence related to beta toxin, NetB, and Staphylococcus pore-forming toxins, but shows functional differences.
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灌注芽孢杆菌三角毒素是与β毒素，NETB和葡萄球菌形成孔形成毒素有关的序列，但显示功能差异。
DOI：
10.1371/journal.pone.0003764
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发表时间：
2008
期刊：
影响因子：
3.7
通讯作者：
Popoff MR
中科院分区：
文献类型：
--
作者：
Manich M;Knapp O;Gibert M;Maier E;Jolivet-Reynaud C;Geny B;Benz R;Popoff MR
产气荚膜梭菌产生许多毒素，这些毒素是导致人类和动物严重疾病的原因。δ毒素是由许多C. C型产气荚膜杆菌和可能的B型菌株。δ毒素被表征为对在其膜中表达神经节苷脂GM 2的细胞具有细胞毒性。在这里，我们报告的Delta毒素的遗传特性和其在脂质双层中的成孔活性。δ毒素由318个氨基酸组成，其28个N-末端氨基酸对应于信号肽。分泌的Delta毒素（290个氨基酸; 32619 Da）是碱性蛋白（pI 9.1），其显示与C.产气荚膜杆菌β毒素（43%的同一性），与C.与产气荚膜杆菌NetB（40%同一性），并且在较小程度上与金黄色葡萄球菌α毒素和白细胞毒素。重组δ毒素显示出与GM 2结合的偏好，与不与神经节苷脂结合的β毒素相反。对绵羊红细胞具有溶血性，对HeLa细胞具有细胞毒性。在人工二植烷酰磷脂酰胆碱膜中，δ和β毒素形成通道。δ毒素形成的通道电导在1 M KCl和20 mV膜电位下约为100 pS至1 nS以上，高于β毒素形成的通道电导，且分布较广。零电流膜电位测量和单通道实验的结果表明，δ毒素形成轻微的阴离子选择性通道，而β毒素通道在相同的条件下表现出对阳离子的偏好。C.产气荚膜杆菌δ毒素与C.产气荚膜杆菌β和NetB毒素，以及S.金黄色葡萄球菌α溶血素和白细胞毒素，但在脂质双层中表现出不同的通道特性。与β毒素相反，δ毒素识别GM 2作为受体并形成阴离子选择性通道。
Clostridium perfringens produces numerous toxins, which are responsible for severe diseases in man and animals. Delta toxin is one of the three hemolysins released by a number of C. perfringens type C and possibly type B strains. Delta toxin was characterized to be cytotoxic for cells expressing the ganglioside GM2 in their membrane. Here we report the genetic characterization of Delta toxin and its pore forming activity in lipid bilayers. Delta toxin consists of 318 amino acids, its 28 N-terminal amino acids corresponding to a signal peptide. The secreted Delta toxin (290 amino acids; 32619 Da) is a basic protein (pI 9.1) which shows a significant homology with C. perfringens Beta toxin (43% identity), with C. perfringens NetB (40% identity) and, to a lesser extent, with Staphylococcus aureus alpha toxin and leukotoxins. Recombinant Delta toxin showed a preference for binding to GM2, in contrast to Beta toxin, which did not bind to gangliosides. It is hemolytic for sheep red blood cells and cytotoxic for HeLa cells. In artificial diphytanoyl phosphatidylcholine membranes, Delta and Beta toxin formed channels. Conductance of the channels formed by Delta toxin, with a value of about 100 pS to more than 1 nS in 1 M KCl and a membrane potential of 20 mV, was higher than those formed by Beta toxin and their distribution was broader. The results of zero-current membrane potential measurements and single channel experiments suggest that Delta toxin forms slightly anion-selective channels, whereas the Beta toxin channels showed a preference for cations under the same conditions. C. perfringens Delta toxin shows a significant sequence homolgy with C. perfringens Beta and NetB toxins, as well as with S. aureus alpha hemolysin and leukotoxins, but exhibits different channel properties in lipid bilayers. In contrast to Beta toxin, Delta toxin recognizes GM2 as receptor and forms anion-selective channels.
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DOI：
10.1016/s0925-4439(99)00026-5
发表时间：
1999-05-31
影响因子：
6.2
作者：
Nagahama, M;Kihara, A;Sakurai, J
通讯作者：
Sakurai, J
影响因子：
2.7
作者：
Geny, Blandine;Popoff, Michel R.
通讯作者：
Popoff, Michel R.
DOI：
10.1016/0005-2736(89)90002-3
发表时间：
1979-01-01
期刊：
BIOCHIMICA ET BIOPHYSICA ACTA
影响因子：
--
作者：
BENZ, R;JANKO, K;LAUGER, P
通讯作者：
LAUGER, P
影响因子：
2.9
作者：
Heuck, AP;Tweten, RK;Johnson, AE
通讯作者：
Johnson, AE
影响因子：
2.3
作者：
JOLIVETREYNAUD, C;ESTRADA, J;CHEDID, L
通讯作者：
CHEDID, L