Identification and mapping of Rpi-blb4 in diploid wild potato species Solanum bulbocastanum

Identification and mapping of Rpi-blb4 in diploid wild potato species Solanum bulbocastanum
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二倍体野生马铃薯品种 Solanum tubecastanum 中 Rpi-blb4 的鉴定和定位

DOI:
10.1016/j.cj.2023.08.005
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发表时间:
2023
期刊:
The Crop Journal
影响因子:
--
通讯作者:
Li J
Li J
中科院分区:
--
文献类型:
--
作者:
Li J

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在造成爱尔兰马铃薯饥荒170多年后,晚疫病仍然被认为是最具破坏性的作物疾病之一。商业马铃薯育种工作依赖于自然抗源来保护作物免受快速进化的晚疫病病原菌的侵染。我们鉴定并定位了一个新的广谱抗病基因。来自野生二倍体马铃薯品种球茎茄子的寄生虫。利用诊断抗性基因富集性测序(DRenSeq)来确认鉴定的抗性的唯一性。基于RenSeq和GenSeq的抗性作图,称为Rpi-blb4,结合重组筛选,定位了马铃薯5号染色体的抗性基因座。该区间跨度约为2.3Mb,对应于DM参考基因组位置11.25Mb和13.56Mb。
More than 170 years after causing the potato famine in Ireland, late blight is still considered one of the most devastating crop diseases. Commercial potato breeding efforts depend on natural sources of resistance to protect crops from the rapidly evolving late blight pathogen,Phytophthora infestans.We have identified and mapped a novel broad-spectrum disease resistance gene effective againstP. infestansfrom the wild, diploid potato speciesSolanum bulbocastanum. Diagnostic resistance gene enrichment sequencing (dRenSeq) was used to confirm the uniqueness of the identified resistance. RenSeq and GenSeq-based mapping of the resistance, referred to asRpi-blb4, alongside recombinant screening, positioned the locus responsible for the resistance to potato chromosome 5. The interval spans approximately 2.3 Mb and corresponds to the DM reference genome positions of 11.25 and 13.56 Mb.
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