Tissue‐specific expansion of NKT and CD5+B cells at the onset of autoimmune disease in (NZB×NZW)F1 mice
Tissue‐specific expansion of NKT and CD5+B cells at the onset of autoimmune disease in (NZB×NZW)F1 mice
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(NZB×NZW)F1 小鼠自身免疫性疾病发作时 NKT 和 CD5+B 细胞的组织特异性扩增
DOI：
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发表时间：
2002
影响因子：
5.4
通讯作者：
T. Abo
中科院分区：
文献类型：
--
作者：
S. Morshed;K. Mannoor;R. Halder;H. Kawamura;M. Bannai;H. Sekikawa;Hisami Watanabe;T. Abo
在自身免疫易感（NZ B ×NZW）F1（NZ B/W F1）小鼠各免疫器官中寻找自然杀伤T（NKT）细胞和CD 5 +B细胞。发病后（30周龄），肝脏、脾脏和腹腔中淋巴细胞的数量增加，而胸腺细胞的数量减少。 肝脏和腹腔淋巴细胞亚群变化显著，肝脏IL-2 R β+TCRα βint细胞和腹腔CD 5 +B220+细胞扩增。肝脏中TCRα βint细胞以NK 1.1+为主，腹腔中CD 5 +B细胞以CD 1d+为主。随着疾病的进展，蛋白尿在NZB/WF 1小鼠中变得突出。与此同时，肝脏中NKT细胞的比例略有下降，但其绝对数量在该器官中保持在较高水平。这些NKT细胞为CD 4+，并使用Vα 14 J α281的不变链作为TCRα。血清中抗肝细胞胞浆抗体和变性DNA抗体的出现反映了CD 5 +B细胞的升高。虽然已知NKT细胞在某些自身免疫小鼠中是免疫调节细胞，但本研究结果提出了NKT细胞以及CD 5 +B细胞可能与NZ B/W F1小鼠中自身免疫性疾病的发作相关的可能性。事实上，当给予α-半乳糖神经酰胺或将活性NKT细胞转移到年轻的F1小鼠时，F1小鼠中的NKT细胞具有诱导自身免疫样炎症的高潜力。
Natural killer T (NKT) cells and CD5+B cells were searched for in various immune organs of autoimmune prone (NZB×NZW)F1 (NZB/W F1) mice. The number of lymphocytes increased in the liver, spleen, and peritoneal cavity after the onset of disease (at the age of 30 weeks) while the number of thymocytes decreased at that time. Prominent changes of lymphocyte subsets were seen in the liver and peritoneal cavity, namely, expansion of IL‐2Rβ+TCRα βint cells in the liver and of CD5+B220+ cells in the peritoneal cavity. The majority of TCRα βint cells in the liver were NK1.1+, and CD5+B cells in the peritoneal cavity were CD1d+. Proteinuria became prominent in NZB/W F1 mice with the progression of disease. In parallel with this progression, the proportion of NKT cells decreased slightly in the liver, but their absolute number remained at a high level in this organ. These NKT cells were CD4+ and used an invariant chain of Vα14Jα281 for TCRα. Reflecting the elevation of CD5+B cells, autoantibodies against hepatocyte cytoplasmand denatured DNA were detected in sera. Although NKT cells are known to be immunoregulatory cells in some autoimmune mice, the present results raise the possibility that NKT cells as well as CD5+B cells might be associated with the onset of autoimmune diseases in NZB/W F1 mice. Indeed, NKT cells in F1 mice had a high potential to induce autoimmune‐like inflammationwhen α–galactosylceramide was administered or when active NKT cells were transferred into young F1 mice.
影响因子：
4.4
作者：
Masahiko Amano;Nicole Baumgarth;M. Dick;L. Brossay;Mitchell Kronenberg;Leonard A. Herzenberg;S. Strober
通讯作者：
Masahiko Amano;Nicole Baumgarth;M. Dick;L. Brossay;Mitchell Kronenberg;Leonard A. Herzenberg;S. Strober
小鼠 CD1 呈递肽抗原需要内体定位和蛋白质抗原加工。
DOI：
10.1073/pnas.95.24.14314
发表时间：
1998
影响因子：
11.1
作者：
Tangri,S;Brossay,L;Burdin,N;Lee,DJ;Corr,M;Kronenberg,M
通讯作者：
Kronenberg,M