Genetic manipulation in pigs.
Genetic manipulation in pigs.
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DOI：
10.1097/mot.0b013e3283292549
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发表时间：
2009-04
影响因子：
2.2
通讯作者：
Galli C
中科院分区：
文献类型：
--
作者：
Sachs DH;Galli C
基因工程领域的最新发展使得从一个物种向另一个物种添加、删除或交换基因成为可能。这项技术与异种移植领域特别相关，在异种移植领域，消除物种特异性差异可能决定器官移植的成功或失败。本文综述了转基因动物的技术和应用的发展。利用现有技术对猪进行遗传修饰，以及开发新的新兴技术，包括酶工程和使用siRNA，都取得了进展。转基因动物的应用提供了证据，表明转基因猪有潜力克服以前阻碍这一领域的生理和免疫障碍。使用GalT-KO动物作为供体已经显示出异种移植物存活率的显著改善。用于猪的基因工程的技术已经针对避免对物种特异性抗原的天然存在的细胞和抗体应答。来自基因工程动物的器官在非人类灵长类动物中的存活率显著提高，特别是在旨在诱导耐受性的方案中，可能是通过避免对新抗原的免疫。
Recent developments in the field of genetic engineering have made it possible to add, delete or exchange genes from one species to another. This technology has special relevance to the field of xenotransplantation, in which the elimination of a species-specific disparity could make the difference between success or failure of an organ transplant. This review focuses on developments in both the techniques and applications of genetically modified animals. Advances have been made using existing techniques for genetic modifications of swine and in the development of new, emerging technologies, including enzymatic engineering and the use of siRNA. Applications of the modified animals have provided evidence that genetically modified swine have the potential to overcome both physiologic and immunologic barriers that have previously impeded this field. Use of GalT-KO animals as donors have shown marked improvements in xenograft survivals. Techniques for genetic engineering of swine have been directed toward avoiding naturally existing cellular and antibody responses to species-specific antigens. Organs from genetically engineered animals have enjoyed markedly improved survivals in non-human primates, especially in protocols directed toward the induction of tolerance, presumably by avoiding immunization to new antigens.
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影响因子：
3.7
作者：
Jónsson SR;LaRue RS;Stenglein MD;Fahrenkrug SC;Andrésdóttir V;Harris RS
通讯作者：
Harris RS
影响因子：
3.9
作者：
Dieckhoff, Britta;Petersen, Bjoern;Denner, Joachim
通讯作者：
Denner, Joachim
影响因子：
64.8
作者：
HAMMER, RE;PURSEL, VG;BRINSTER, RL
通讯作者：
BRINSTER, RL
影响因子：
1.8
作者：
Kurome, Mayuko;Ishikawa, Takayuki;Nagashima, Hiroshi
通讯作者：
Nagashima, Hiroshi
影响因子：
1.5
作者：
Diaz-Roman, Tomas M.;Manez, Rafael;Domenech, Nieves
通讯作者：
Domenech, Nieves