Homologous recombination between transfected DNAs
Homologous recombination between transfected DNAs
复制标题
转染 DNA 之间的同源重组
DOI：
10.1128/mcb.3.9.1680-1685.1983
复制
发表时间：
1983
影响因子：
5.3
通讯作者：
J. Hassell
中科院分区：
文献类型：
--
作者：
B. Pomerantz;M. Naujokas;J. Hassell
对转染到Rat-1细胞中的多瘤病毒-质粒重组分子的命运和结构的广泛分析揭示，DNA通常以头对尾串联排列整合到转化的细胞DNA中。这独立于转化DNA的复制能力而发生，并且通过在转染期间使用大量DNA而促进。这些观察结果使我们认为，头-尾串联是通过转染的DNA之间在整合到细胞DNA内之前或之后的同源重组形成的。为了验证这一假设，我们测量了多瘤病毒转化基因中携带不同病变的突变型、非转化型、重组质粒DNA对的转化活性。结果表明，虽然单个突变DNA不能转化，但用成对的突变DNA转染导致高频率的转化细胞的形成。此外，成对突变DNA中病变之间的距离与其转化活性之间存在直接关系。最后，整合的重组质粒DNA和独立的转化细胞内的病毒蛋白质的结构的分析证明，重组发生在突变体基因组之间，以产生野生型转化基因。
An extensive analysis of the fate and structure of polyomavirus-plasmid recombinant molecules transfected into Rat-1 cells has revealed that the DNA often becomes integrated within transformed cell DNA in a head-to-tail tandem arrangement. This occurs independently of the replicative capacity of the transforming DNA and is facilitated by the use of large quantities of DNA during transfection. These observations have led us to suggest that head-to-tail tandems are formed by homologous recombination between transfected DNAs either before or after integration within cellular DNA. To test this hypothesis, we have measured the transforming activity of pairs of mutant, nontransforming, recombinant plasmid DNAs that carry different lesions in the transforming gene of polyomavirus. The results show that, although the individual mutant DNAs are incapable of transformation, transfection with pairs of mutant DNAs leads to the formation of transformed cells at high frequency. Moreover, there is a direct relationship between the distance between the lesions in pairs of mutant DNAs and their transforming activity. Finally, analyses of the structures of integrated recombinant plasmid DNAs and the viral proteins within independent transformed cells prove that recombination occurs between the mutant genomes to generate a wild-type transforming gene.
影响因子：
56.9
作者：
C. Miller;H. Temin
通讯作者：
C. Miller;H. Temin
仅含有部分早期区域的克隆多瘤病毒 DNA 片段对大鼠胚胎成纤维细胞的转化。
DOI：
10.1073/pnas.77.7.3978
发表时间：
1980
影响因子：
11.1
作者：
Hassell,JA;Topp,WC;Rifkin,DB;Moreau,PE
通讯作者：
Moreau,PE
多瘤病毒基因组的整合和切除。
DOI：
10.1101/sqb.1980.044.01.064
发表时间：
1980
期刊：
Cold Spring Harbor symposia on quantitative biology
影响因子：
--
作者：
Basilico,C;Zouzias,D;Della-Valle,G;Gattoni,S;Colantuoni,V;Fenton,R;Dailey,L
通讯作者：
Dailey,L