Comprehensive mapping of the Helicobacter pylori NikR regulon provides new insights in bacterial nickel responses
Comprehensive mapping of the Helicobacter pylori NikR regulon provides new insights in bacterial nickel responses
复制标题
幽门螺杆菌 NikR 调节子的全面图谱为细菌镍反应提供了新见解
作者：
A. Vannini;E. Pinatel;P. E. Costantini;Simone Pelliciari;Davide Roncarati;S. Puccio;G. De Bellis;C. Peano;A. Danielli
镍稳态对于致病菌和尿素分解菌是重要的，它们使用这种金属离子作为酶辅助因子。例如，在人类病原体幽门螺杆菌中，镍摄取和金属酶中的掺入之间的最佳平衡是宿主定殖的基础。镍还用作辅助因子来调节NikR调节剂的DNA结合，所述NikR调节剂控制参与许多细菌中的镍运输或感染的基因的转录。因此，对NikR调节的系统表征有很大的兴趣。这里我们使用H。pylori作为整合RNA-seq和ChIP-seq数据的模型，证明NikR不仅调节金属离子转运蛋白，而且调节毒力因子、非编码RNA以及响应镍刺激的毒素-抗毒素系统。总之，研究结果为H. pylori，并有助于了解其他细菌对镍的反应。
Nickel homeostasis is important for pathogenic and ureolytic bacteria, which use this metal ion as enzymatic cofactor. For example, in the human pathogen Helicobacter pylori an optimal balance between nickel uptake and incorporation in metallo-enzymes is fundamental for colonization of the host. Nickel is also used as cofactor to modulate DNA binding of the NikR regulator, which controls transcription of genes involved in nickel trafficking or infection in many bacteria. Accordingly, there is much interest in a systematic characterization of NikR regulation. Herein we use H. pylori as a model to integrate RNA-seq and ChIP-seq data demonstrating that NikR not only regulates metal-ion transporters but also virulence factors, non-coding RNAs, as well as toxin-antitoxin systems in response to nickel stimulation. Altogether, results provide new insights into the pathobiology of H. pylori and contribute to understand the responses to nickel in other bacteria.
DOI：
10.1039/c1mt00063b
发表时间：
2011-11
期刊：
Metallomics : integrated biometal science
影响因子：
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作者：
Macomber L;Hausinger RP
通讯作者：
Hausinger RP