Intramuscular delivery of mIL-12 gene reduces the expression of CD44/CD49d on pulmonary leucocytes and inhibits ovalbumin-induced airway hyperreactivity
Intramuscular delivery of mIL-12 gene reduces the expression of CD44/CD49d on pulmonary leucocytes and inhibits ovalbumin-induced airway hyperreactivity
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肌肉注射 mIL-12 基因可降低肺白细胞上 CD44/CD49d 的表达并抑制卵清蛋白诱导的气道高反应性
DOI：
10.1007/s00011-007-7042-1
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发表时间：
2008
影响因子：
6.7
通讯作者：
W. Chen
中科院分区：
文献类型：
--
作者：
H. Li;Q. Xie;H. Wang;C. Shao;W. Chen
目的：探讨小鼠白细胞介素12（mIL-12）基因对肺白细胞表面CD 44和CD 49 d表达的影响。结果：mIL-12质粒可显著降低哮喘小鼠气道高反应性、肺白细胞表面CD 44和CD 49 d的表达、炎性细胞浸润、Th 2细胞因子的产生和杯状细胞增生（P < 0.05）。CD 44 +/CD 49 d+与炎性细胞数、杯状细胞增生、IL-4、IL-5水平呈正相关（P < 0.01）。结论：CD 44 +/CD 49 d+可能参与了mIL-12基因对哮喘的预防作用。
Abstract.Objective:To investigate the effects of murine IL-12 (mIL-12) gene on the expression of CD44 and CD49d on pulmonary leukocytes.Material and methods:BALB/c mice (n = 24) were sensitized to ovalbumin (OVA) by intraperitoneal injection and challenged with OVA aerosol for 7 consecutive days. mIL-12 plasmid was then intramuscularly administered in eight BALB/c mice every second day for three times during OVA challenge.Results:Administration of mIL-12 plasmid significantly reduced airway hyperreactivity, the expression of CD44 and CD49d on pulmonary leukocytes, pulmonary infiltration of inflammatory cells, Th2 cytokines production and goblet cell hyperplasia (P < 0.05). Importantly, the percentages of CD44+/CD49d+ leucocytes showed positive correlations with numbers of inflammatory cells, goblet cells hyperplasia and levels of IL-4 and IL-5 (P < 0.01).Conclusions:Our study suggests that CD44 and CD49d might contribute to the preventive effects of mIL-12 gene in asthma.
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DOI：
10.1165/ajrcmb.16.4.9115759
发表时间：
1997-04-01
影响因子：
6.4
作者：
Temann, UA;Prasad, B;Rankin, JA
通讯作者：
Rankin, JA
影响因子：
4.4
作者：
G. Lack;K. Bradley;Eckard Hamelmann;H. Renz;J. Loader;D. Leung;G. Larsen;E. Gelfand
通讯作者：
G. Lack;K. Bradley;Eckard Hamelmann;H. Renz;J. Loader;D. Leung;G. Larsen;E. Gelfand
DOI：
10.1165/ajrcmb.18.6.3159
发表时间：
1998-06-01
影响因子：
6.4
作者：
Matsumoto, K;Appiah-Pippim, J;Bochner, BS
通讯作者：
Bochner, BS
影响因子：
56.9
作者：
DeGrendele, HC;Estess, P;Siegelman, MH
通讯作者：
Siegelman, MH