Brain NaK-ATPases in mice differentially sensitive to alcohols.
Brain NaK-ATPases in mice differentially sensitive to alcohols.
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小鼠脑 NaK-ATP 酶对酒精差异敏感。
DOI：
10.1111/j.1530-0277.1984.tb05685.x
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发表时间：
1984
期刊：
影响因子：
--
通讯作者：
Collins,AC
中科院分区：
文献类型：
--
作者：
Marks,MJ;Smolen,A;Collins,AC
在从LS和SS小鼠皮质制备的匀浆颗粒部分中研究了NaK-ATP酶同工酶，这两种小鼠系已选择性繁殖以对乙醇产生差异反应。除了哇巴因不敏感的Mg-ATP酶外，哇巴因剂量反应曲线表明脑中存在两种NaK-ATP酶活性，对哇巴因具有不同的敏感性。这些被指定为低K（4 × 10 - 7 M）和高K1（2 × 10 - 4 M）。乙醇对ATP酶的抑制作用不同：哇巴因不敏感活性对乙醇抑制的敏感性低于两种哇巴因敏感活性。低K1（脑特异性）NaK-ATP酶比高K1活性更敏感。然而，乙醇抑制所有三个组件的ATP酶活性以相同的方式在两个小鼠系。与高K1活性相比，低K1活性对热变性和对羟基苯甲酸汞变性也更不稳定。这些措施在LS和SS线路之间没有区别。从LS和SS小鼠获得的皮质匀浆的颗粒部分的SDS-聚丙烯酰胺电泳显示，在两个品系中存在两条具有相似32 P标记的蛋白条带。考虑到电泳模式和热或对羟基苯甲酸汞抑制是相同的，ATP酶活性的差异或乙醇的抑制似乎不太可能是LS和SS小鼠对乙醇的不同反应的原因。
The isozymes of NaK‐ATPase were studied in the particulate fraction of homogenates prepared from cortex of LS and SS mice, two lines of mice that have been selectively bred for differential response to ethanol. In addition to a ouabain‐insensitive Mg‐ATPase, ouabain dose‐response curves have suggested the presence, in brain, of two NaK‐ATPase activities with different sensitivities to ouabain. These are designated low K, (4 times 10‐7M) and high K1(2 times 10‐4M). Ethanol differentially inhibited the ATPases: The ouabain‐insensitive activity was less sensitive to ethanol inhibition than were the two ouabain‐inhibitable activities. The low K1(brain specific) NaK‐ATPase was more sensitive than was the high K, activity. However, ethanol inhibited all three components of the ATPase activity in an identical fashion in the two mouse lines. The low K, activity was also more labile to thermal and p‐hydroxymercurobenzoate denaturabon than was the high K1activity. These measures did not differ between the LS and SS lines. SDS‐polyacrylamide electrophoresis of particulate fraction of cortical homogenates obtained from LS and SS mice revealed the presence of two protein bands with similar32P labeling in both lines. Given that etectrophoretic pattern and heat or p‐hydroxymercurobenzoate inhibition were identical, it seems unlikely that differences in ATPase activity, or inhibition by ethanol, are responsible for the different response of LS and SS mice to ethanol.
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影响因子：
6.1
作者：
M. Marks;N. Seeds
通讯作者：
M. Marks;N. Seeds
酒精抑制作用中性别相关和基因决定差异的机制 *
影响因子：
5.2
作者：
A. C. Collins;M. Lebsack;T. N. Yeager
通讯作者：
T. N. Yeager
DOI：
--
发表时间：
1983
期刊：
Pharmacology, Biochemistry and Behavior
影响因子：
--
作者：
C. Guerri;S. Grisolía
通讯作者：
S. Grisolía
重新聚集的脑细胞培养物中离子代谢的发展
影响因子：
4.7
作者：
M. Marks;N. Seeds
通讯作者：
N. Seeds
(Na -K )-ATPase 的两个明显不同的哇巴因结合位点。
DOI：
--
发表时间：
1972
期刊：
Biochimica et Biophysica Acta
影响因子：
--
作者：
K. Taniguchi;S. Iida
通讯作者：
S. Iida