Three-Dimensional Printing of Cell Exclusion Spacers (CES) for Use in Motility Assays.
Three-Dimensional Printing of Cell Exclusion Spacers (CES) for Use in Motility Assays.
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DOI：
10.1007/s11095-018-2431-4
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发表时间：
2018-06-04
影响因子：
3.7
通讯作者：
Alexander JS
中科院分区：
文献类型：
--
作者：
Boyer CJ;Ballard DH;Yun JW;Xiao AY;Weisman JA;Barzegar M;Alexander JS
Cell migration/invasion assays are widely used in commercial drug discovery screening. 3D printing enables the creation of diverse geometric restrictive barrier designs for use in cell motility studies, permitting on-demand assays. Here, the utility of 3D printed cell exclusion spacers (CES) was validated as a cell motility assay. A novel CES fit was fabricated using 3D printing and customized to the size and contour of 12 cell culture plates including 6 well plates of basal human brain vascular endothelial (D3) cell migration cells compared with 6 well plates with D3 cells challenged with 1uM cytochalasin D (Cyto-D), an F-actin anti-motility drug. Control and Cyto-D treated cells were monitored over 3 days under optical microscopy. Day 3 cell migration distance for untreated D3 cells was 1515.943μm ± 10.346μm compared to 356.909μm ± 38.562μm for the Cyto-D treated D3 cells (p < 0.0001). By day 3, untreated D3 cells reached confluency and completely filled the original voided spacer regions, while the Cyto-D treated D3 cells remained significantly less motile. Cell migration distances were significantly reduced by Cyto-D, supporting the use of 3D printing for cell exclusion assays. 3D printed CES have great potential for studying cell motility, migration/invasion, and complex multi-cell interactions.
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Decker SJ