Chromatographic fingerprint and the simultaneous determination of five bioactive components of geranium carolinianum L. water extract by high performance liquid chromatography.
Chromatographic fingerprint and the simultaneous determination of five bioactive components of geranium carolinianum L. water extract by high performance liquid chromatography.
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DOI：
10.3390/ijms12128740
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发表时间：
2011
影响因子：
5.6
通讯作者：
Liu LF
中科院分区：
文献类型：
--
作者：
Wu QY;Zhou Y;Jin X;Guan Y;Xu M;Liu LF
建立了一种简便、灵敏的HPLC指纹图谱分析方法，并同时测定了没食子酸、柯里拉京、短叶苏木酸甲酯、鞣花酸和芦丁5种成分的含量，用于评价和控制卷柏药材的质量。水提取物。通过比较老鹳草中主要单宁老鹳草苷的水解效率，确定了水回流提取老鹳草苷的方法。采用HPLC法分析水提取物，以0.1%磷酸水溶液（v/v）和乙腈为移动的流动相，在65 min内进行梯度洗脱。在274 nm UV波长处检测化合物。在指纹图谱分析中，选取17个峰作为特征峰，对南京郊区不同产地的样品进行相似性评价。相似性相关系数均大于0.993。在定量分析中，5种标记物在检测范围内线性关系良好（R > 0.9991），平均回收率在97.2-101.7%之间，相对标准偏差（RSD）均小于4.50%。5种标记物的总含量在44.28 ~ 71.84mg/g之间。该方法对进一步开发G.卡罗利纳木提取物和制剂。
A simple and sensitive HPLC method has been developed in combination with fingerprint analysis and simultaneous determination of five markers, namely gallic acid, corilagin, methyl brevifolincarboxylate, ellagic acid and rutin for evaluation and quality control of Geranium carolinianum L. water extract. Extraction methods were optimized by comparing the hydrolysis efficiency of geraniin, a major tannin of the herb, resulting in the method of extraction with water under reflux. Water extracts were analyzed by HPLC, with a mobile phase of 0.1% aqueous phosphoric acid (v/v) and acetonitrile in a gradient program within 65 min. Compounds were detected at 274 nm UV wavelength. For fingerprint analysis, 17 peaks were selected as the characteristic peaks to evaluate the similarities of different samples collected from the suburb of Nanjing. The correlation coefficients of similarity were greater than 0.993. In quantitative analysis, the five selected markers showed good regression (R > 0.9991) within test ranges, and the average recoveries were between 97.2–101.7% and their RSD values were less than 4.50%. The total contents of the five markers varied from 44.28 to 71.84 mg/g. The method can be very useful for further development of G. carolinianum L. extracts and preparations.
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