IR spectroscopic studies of major cellular components. III. Hydration of protein, nucleic acid, and phospholipid films.
IR spectroscopic studies of major cellular components. III. Hydration of protein, nucleic acid, and phospholipid films.
复制标题
主要细胞成分的红外光谱研究。
DOI：
10.1002/bip.10416
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发表时间：
2003
期刊：
影响因子：
--
通讯作者：
Diem,Max
中科院分区：
文献类型：
--
作者：
Pevsner,Alex;Diem,Max
The IR absorption spectra of protein, DNA, RNA, and phospholipid films as a function of the water content are reported. We find that the hydration of protein films affects the peak intensity of amide I and amide II bands and the shape of the amide III band. For nucleic acids, the symmetric (νSPO) and antisymmetric (νASPO) stretching vibrations of the phosphate linkage are the most affected by hydration, because both intensity changes and frequency shifts are observed. The spectra of phospholipid films are also sensitive to hydration, and they exhibit changes in the peak intensities and frequencies of both νSPO and νASPO vibrations. We interpret the spectral differences between water saturated and dried films both in terms of structural changes and the change in the local dielectric in the vicinity of the polar and solvent exposed groups. In addition, we observe that the most significant change in the absorption intensity, frequency, and shape of the water sensitive vibrations occurs at high hydration levels. The principal component analysis of hydration results and the kinetics of water removal from sample films are also discussed. In addition, protein spectra acquired using film and KBr pellet sampling techniques are compared. © 2003 Wiley Periodicals, Inc. Biopolymers (Biospectroscopy), 2003
主要细胞成分的红外光谱研究。
DOI：
--
发表时间：
2001
期刊：
影响因子：
--
作者：
A. Pevsner;M. Diem
通讯作者：
M. Diem
DOI：
--
发表时间：
1963
期刊：
影响因子：
--
作者：
M. Falk;K. A. Hartman;R. Lord
通讯作者：
R. Lord
DNA 分子构型的红外研究
DOI：
--
发表时间：
1961
期刊：
影响因子：
--
作者：
E. Bradbury;W. Price;G. Wilkinson
通讯作者：
G. Wilkinson