Environmental DNA reveals tropical shark diversity in contrasting levels of anthropogenic impact.
Environmental DNA reveals tropical shark diversity in contrasting levels of anthropogenic impact.
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DOI：
10.1038/s41598-017-17150-2
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发表时间：
2017-12-04
影响因子：
4.6
通讯作者：
Mariani S
中科院分区：
文献类型：
--
作者：
Bakker J;Wangensteen OS;Chapman DD;Boussarie G;Buddo D;Guttridge TL;Hertler H;Mouillot D;Vigliola L;Mariani S
鲨鱼是魅力十足的掠食者，在大多数海洋食物网中发挥着关键作用。它们被证明易受开发，最近已成为海洋保护的旗舰物种。然而，评估和监测海洋环境中这类移动的物种的分布和丰度仍然具有挑战性，往往是侵入性的，而且需要大量资源。在这里，我们试点一种新的，快速的和非侵入性的环境DNA（eDNA）metabarcoding的方法，专门针对推断鲨鱼的存在，多样性和eDNA读取丰富的热带栖息地。我们确定了至少21种鲨鱼，从加勒比海和太平洋珊瑚海的水样，其多样性和读丰富的地理格局与人为压力和保护努力的地理差异相吻合。我们证明，eDNA元条码可以有效地用于研究鲨鱼的多样性。这一领域的进一步发展有可能大大提高我们评估和监测难以捉摸的海洋捕食者的能力，并改善保护战略。
Sharks are charismatic predators that play a key role in most marine food webs. Their demonstrated vulnerability to exploitation has recently turned them into flagship species in ocean conservation. Yet, the assessment and monitoring of the distribution and abundance of such mobile species in marine environments remain challenging, often invasive and resource-intensive. Here we pilot a novel, rapid and non-invasive environmental DNA (eDNA) metabarcoding approach specifically targeted to infer shark presence, diversity and eDNA read abundance in tropical habitats. We identified at least 21 shark species, from both Caribbean and Pacific Coral Sea water samples, whose geographical patterns of diversity and read abundance coincide with geographical differences in levels of anthropogenic pressure and conservation effort. We demonstrate that eDNA metabarcoding can be effectively employed to study shark diversity. Further developments in this field have the potential to drastically enhance our ability to assess and monitor elusive oceanic predators, and lead to improved conservation strategies.
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影响因子：
3.7
作者：
Civade R;Dejean T;Valentini A;Roset N;Raymond JC;Bonin A;Taberlet P;Pont D
通讯作者：
Pont D
影响因子：
3.8
作者：
Gilman, Eric;Clarke, Shelley;Werner, Tim
通讯作者：
Werner, Tim
影响因子：
3.7
作者：
Cinner JE;Huchery C;Darling ES;Humphries AT;Graham NA;Hicks CC;Marshall N;McClanahan TR
通讯作者：
McClanahan TR
影响因子：
4.4
作者：
Ficetola GF;Coissac E;Zundel S;Riaz T;Shehzad W;Bessière J;Taberlet P;Pompanon F
通讯作者：
Pompanon F
影响因子：
3.7
作者：
Guttridge, Tristan L.;Bergmann, Maurits P. M. Van Zinnicq;Gruber, Samuel H.
通讯作者：
Gruber, Samuel H.