Immunocytochemical assay of estrogen receptors in endometrial carcinoma with monoclonal antibodies. Comparison with biochemical assay
Immunocytochemical assay of estrogen receptors in endometrial carcinoma with monoclonal antibodies. Comparison with biochemical assay
复制标题
使用单克隆抗体对子宫内膜癌中雌激素受体进行免疫细胞化学测定。
作者：
L. Pertschuk;A. Beddoe;L. Gorelic;S. Shain
采用单克隆抗雌激素受体抗体和过氧化物酶抗过氧化物酶技术，通过免疫细胞化学分析从18名女性手术获得的子宫内膜腺癌标本中雌激素受体的分布。结果与生化受体分析进行了比较，其中83%的结果一致。免疫细胞化学方法显示肿瘤细胞和组织受体的异质性明显，阳性组织标本中出现多种核染色模式。这些结果表明，免疫组织学方法将提供许多全新的变量，这些变量最终可能与这种恶性肿瘤的临床和病理特征相关，并且可能被证明在预测临床内分泌反应方面具有价值。
Specimens of endometrial adenocarcinoma, surgically obtained from 18 women, were analyzed for distribution of estrogen receptors by an immunocytochemical assay, employing monoclonal anti‐estrophilin antibodies and the peroxidase‐antiperoxidase technique. Results were compared with biochemical receptor analyses, and were in concordance in 83% of them. Marked tumor cell and tissue receptor heterogeneity were apparent with the immunocytochemical method, and a variety of patterns of nuclear staining in positive tissue samples were revealed. These results indicate that the immunohistologic method will provide a number of entirely new variables that may eventually be correlated with both clinical and pathologic features of this malignancy, and may prove to be of value in the prediction of clinical endocrine response.
DOI：
10.1097/00006254-198510000-00017
发表时间：
1984-11
期刊：
Laboratory investigation; a journal of technical methods and pathology
影响因子：
--
作者：
M. Press;N. Nousek-Goebl;W. King;A. Herbst;G. Greene
通讯作者：
M. Press;N. Nousek-Goebl;W. King;A. Herbst;G. Greene
使用人雌激素单克隆抗体证明人子宫中雌激素受体的免疫细胞化学方法。
DOI：
--
发表时间：
1984
期刊：
Laboratory investigation; a journal of technical methods and pathology
影响因子：
--
作者：
Press,MF;Greene,GL
通讯作者：
Greene,GL