Muscle actin genes and muscle cells in the appendicularian, Oikopleura longicauda: phylogenetic relationships among muscle tissues in the urochordates.
Muscle actin genes and muscle cells in the appendicularian, Oikopleura longicauda: phylogenetic relationships among muscle tissues in the urochordates.
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阑尾 Oikopleura longicauda 中的肌肉肌动蛋白基因和肌肉细胞：尾索动物肌肉组织之间的系统发育关系。
DOI：
10.1002/1097-010x(20000815)288:2
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发表时间：
2000
期刊：
影响因子：
--
通讯作者：
N. Satoh
中科院分区：
文献类型：
--
作者：
A. Nishino;Y. Satou;M. Morisawa;N. Satoh
附肢动物(幼体动物)是浮游的被衣动物。它们一生都有一条尾巴，这标志着附肢动物与其他被囊类动物的明显区别，包括盐类、石龙类、火龙体和海鞘类。我们从附肢动物Oikopleura Longicauda中分离到编码肌型和细胞质类型肌动蛋白亚型的cDNA克隆。与其他脊索肌和细胞质肌动蛋白相比，诊断残基中的氨基酸同源性证实了肌动蛋白亚型的类型。有趣的是，附属肌肌动蛋白亚型序列介于海鞘尾部(幼虫)肌肌动蛋白亚型和体壁(成体)肌肌动蛋白亚型之间。对肌肉肌动蛋白基因的基因组克隆的分析表明，它在编码区只包含一个内含子。它位于一个与其他后口肌动蛋白基因中报道的任何内含子不对应的位置。整装原位杂交表明，肌肉肌动蛋白转录本在尾芽晚期胚胎的尾部肌肉细胞中特异地检测到。在心脏形成的区域也发现了短暂的信号。在成年人中，该基因在尾部肌肉细胞中表达，但在心脏中不表达。结合肌肉组织结构的细胞化学和组织化学研究结果，本研究强调了附属器肌肉组织的特征，并与腹水动物的幼体和成体肌肉进行了比较。
Appendicularians (larvaceans) are planktonic tunicates. They possess a tail throughout their life, which marks a distinct difference between appendicularians and the other tunicate groups, including salps, doliolids, pyrosomes, and ascidians. We isolated cDNA clones encoding muscle-type and cytoplasmic-type actin isoforms from the appendicularian, Oikopleura longicauda. The types of the actin isoforms were confirmed by amino acid identities in the diagnostic residues compared to those of the other chordate muscle and cytoplasmic actins. Interestingly the appendicularian muscle actin isoform sequence has an intermediate feature between the ascidian tail (larval) muscle actin isoform and the body-wall (adult) muscle actin isoform. Analysis of a genomic clone from a muscle actin gene revealed that it contains only one intron in the coding region. It is located at a position that does not correspond to those of any introns reported in other deuterostome actin genes. Whole-mount in situ hybridization shows that the muscle actin transcript is detected specifically in the tail muscle cells of late tailbud-stage embryos. A signal is also found transiently in the region where the heart will form. In adults, the gene is expressed in tail muscle cells but not in the heart. Together with results of cytochemical and histochemical studies demonstrating the structure of muscle tissue, the present study highlights characteristics of appendicularian muscle organization, which are compared with those of the larval and adult muscle of ascidians.
海鞘幼虫发育过程中成体肌肉肌动蛋白基因的异时表达。
DOI：
10.1002/dvg.1020150107
发表时间：
1994
期刊：
Developmental genetics
影响因子：
--
作者：
Swalla,BJ;White,ME;Zhou,J;Jeffery,WR
通讯作者：
Jeffery,WR
人类骨骼α-肌动蛋白基因的核苷酸序列和表达：功能调节域的进化。
影响因子：
4.4
作者：
Taylor,A;Erba,HP;Muscat,GE;Kedes,L
通讯作者：
Kedes,L