Overexpression of Pim-1 in bladder cancer.
Overexpression of Pim-1 in bladder cancer.
复制标题
Pim-1 在膀胱癌中过度表达
DOI：
10.1186/1756-9966-29-161
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发表时间：
2010-12-11
期刊：
影响因子：
--
通讯作者：
Qiu S
中科院分区：
文献类型：
--
作者：
Guo S;Mao X;Chen J;Huang B;Jin C;Xu Z;Qiu S
背景Pim-1是一种丝氨酸-苏氨酸激酶，可促进肿瘤发生过程中的早期转化、细胞增殖和细胞存活。研究表明Pim-1激酶在不同类型的肿瘤中发挥作用，但Pim-1在膀胱癌中的作用尚不清楚。方法采用免疫组化方法检测Pim-1在正常膀胱组织和膀胱癌组织中的表达，并将Pim-1染色评分与临床病理参数进行比较。为了进一步证明Pim-1在膀胱癌中的生物学功能，通过蛋白质印迹和免疫组织化学分析来验证其在5种膀胱癌细胞系中的表达。随后敲低Pim-1的慢病毒编码的小干扰RNA，和Pim-1对膀胱细胞的生存和药物敏感性的影响进行了进一步评估通过集落形成和细胞增殖assays.ResultsWhen相比，正常上皮，Pim-1在膀胱癌上皮过表达，表达水平高于浸润性膀胱癌比非浸润性膀胱癌标本。Pim-1也在我们研究中检测的所有膀胱癌细胞系中检测到。此外，Pim-1的敲低显着抑制膀胱癌细胞的生长，也敏感细胞化疗drugsin vitro.ConclusionsOur结果表明，Pim-1可能在膀胱癌的发生和发展中发挥作用。由于Pim-1也参与膀胱癌细胞的存活和耐药性，Pim-1是膀胱癌靶向治疗的潜在候选者。
BackgroundPim-1 is a serine-threonine kinase which promotes early transformation, cell proliferation and cell survival during tumorigenesis. Several studies have demonstrated that Pim-1 kinase play a role in different cancer types, however, the function of Pim-1 in bladder cancer is poorly understood.MethodsExpression and localization of Pim-1 in human normal and malignant bladder specimens were examined by Immunohistochemistry and Pim-1 staining score was compared with several clinicopathologic parameters. To further demonstrate the biological function of Pim-1 in bladder cancer, its expression was validated in five bladder cancer cell lines by western blot and immunohistochemistry analyses. Subsequent knockdown of Pim-1 was achieved by lentivirus encoding small interfering RNA, and the effect of Pim-1 on bladder cell survival and drug sensitivity were further assessed by colony formation and cell proliferation assays.ResultsWhen compared with normal epithelium, Pim-1 was overexpressed in bladder cancer epithelium, and the expression level was higher in invasive bladder cancer than Non-invasive bladder cancer specimens. Pim-1 was also detected in all the bladder cancer cell lines examined in our study. Moreover, the knockdown of Pim-1 significantly inhibited bladder cancer cell growth and also sensitized cells to chemotherapeutic drugsin vitro.ConclusionsOur results in this study suggest that Pim-1 may play a role in bladder cancer initiation and progression. Since Pim-1 is also involved in bladder cancer cell survival and drug resistance, Pim-1 is a potential candidate for targeted therapy in bladder cancer.
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影响因子：
4
作者：
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影响因子：
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