Mutations in genes downstream of the rpoN gene (encoding σ54) of Klebsiella pneumoniae affect expression from σ54‐dependent promoters
Mutations in genes downstream of the rpoN gene (encoding σ54) of Klebsiella pneumoniae affect expression from σ54‐dependent promoters
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肺炎克雷伯菌 rpoN 基因（编码 σ54）下游基因突变影响 σ54 依赖性启动子的表达
DOI：
10.1111/j.1365-2958.1989.tb00162.x
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发表时间：
1989
影响因子：
3.6
通讯作者：
J. Coppard
中科院分区：
文献类型：
--
作者：
Mike Merrick;J. Coppard
Two open reading frames (ORFs), designated ORF95 and ORF162, downstream of the Klebsiella pneumoniaeσ54 structural gene (rpoN) have been sequenced and shown to encode polypeptides of 12kD and 16kD, respectively. ORFs homologous to ORF95 are present downstream of four out of five rpoN genes sequenced to date from a range of Gram‐negative bacteria, and ORF162 is also conserved, at least in Pseudomonas putida. Chromosomal mutations have been created in each gene using a kan cassette and both have the same phenotype, i.e. they cause an increase in the level of expression from σ54‐dependent promoters. We propose that the products of both genes function to modulate the activity of Eσ54, although a physiological role for these proteins has not yet been identified.
使用 lac 基因融合表征荚膜红假单胞菌中的 nif 调控基因。
影响因子：
3.5
作者：
Kranz,RG;Haselkorn,R
通讯作者：
Haselkorn,R
DOI：
10.1073/pnas.82.22.7525
发表时间：
1985-01-01
影响因子：
11.1
作者：
HIRSCHMAN, J;WONG, PK;KUSTU, S
通讯作者：
KUSTU, S
DOI：
10.1073/pnas.82.24.8453
发表时间：
1985-12-01
影响因子：
11.1
作者：
HUNT, TP;MAGASANIK, B
通讯作者：
MAGASANIK, B