Acid-Sensing Histidine Kinase With a Redox Switch.
Acid-Sensing Histidine Kinase With a Redox Switch.
复制标题
DOI：
10.3389/fmicb.2021.652546
复制
发表时间：
2021
影响因子：
5.2
通讯作者：
Eguchi Y
中科院分区：
文献类型：
--
作者：
Inada S;Okajima T;Utsumi R;Eguchi Y
大肠杆菌中的EvgS/EVGA双组分信号转导系统在中等酸性的pH条件下被激活。一旦激活，这个系统就会诱导一些赋予耐酸性的基因的表达。EvgS组氨酸激酶传感器有一个大的周质结构域，这是感知酸性信号所必需的。此外，我们先前已经提出，EvgS的细胞质连接区也参与了该传感器的激活。细胞质连接区类似于Per-Arnt-Sim(PAS)结构域，它被认为是一个分子传感器，对化学和物理刺激做出反应，并调节不同效应域的活性。我们的EvgS/EVGA报告分析表明，在EvgS激活的中等酸性pH条件下，EvgS只在有氧生长条件下被激活，而在厌氧生长条件下不被激活。使用EvgS突变体的研究表明，即使在厌氧条件下，细胞质PAS结构域的C671A和C683A突变也能激活EvgS。此外，在电子传递链的电子载体中，辅酶Q是激活EvgS所必需的。本研究提出了氧化激活EvgS的模型，并认为胞质PAS结构域是该传感器的中间氧化还原开关。
The EvgS/EvgA two-component signal transduction system in Escherichia coli is activated under mildly acidic pH conditions. Upon activation, this system induces the expression of a number of genes that confer acid resistance. The EvgS histidine kinase sensor has a large periplasmic domain that is required for perceiving acidic signals. In addition, we have previously proposed that the cytoplasmic linker region of EvgS is also involved in the activation of this sensor. The cytoplasmic linker region resembles a Per-ARNT-Sim (PAS) domain, which is known to act as a molecular sensor that is responsive to chemical and physical stimuli and regulates the activity of diverse effector domains. Our EvgS/EvgA reporter assays revealed that under EvgS-activating mildly acidic pH conditions, EvgS was activated only during aerobic growth conditions, and not during anaerobic growth. Studies using EvgS mutants revealed that C671A and C683A mutations in the cytoplasmic PAS domain activated EvgS even under anaerobic conditions. Furthermore, among the electron carriers of the electron transport chain, ubiquinone was required for EvgS activation. The present study proposes a model of EvgS activation by oxidation and suggests that the cytoplasmic PAS domain serves as an intermediate redox switch for this sensor.
登录
查看更多内容
影响因子：
1.6
作者：
Itou, Junji;Eguchi, Yoko;Utsumi, Ryutaro
通讯作者：
Utsumi, Ryutaro
影响因子：
3.2
作者：
Eguchi, Yoko;Utsumi, Ryutaro
通讯作者：
Utsumi, Ryutaro
通过使用PHOS-TAG SDS-PAGE，对细菌三方传感器激酶的多步磷酸转移信号的顺式和反式模式的验证。
影响因子：
3.7
作者：
Kinoshita-Kikuta E;Kinoshita E;Eguchi Y;Koike T
通讯作者：
Koike T
影响因子：
4.3
作者：
Borisov, Vitaliy B.;Gennis, Robert B.;Hemp, James;Verkhovsky, Michael I.
通讯作者：
Verkhovsky, Michael I.
影响因子：
3.2
作者：
Bekker, Martijn;Alexeeva, Svetlana;Hellingwerf, Klaas
通讯作者：
Hellingwerf, Klaas