Protective effects of resveratrol against UVA-induced damage in ARPE19 cells.
Protective effects of resveratrol against UVA-induced damage in ARPE19 cells.
复制标题
白藜芦醇针对UVA诱导的ARPE19细胞损伤的保护作用。
DOI：
10.3390/ijms16035789
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发表时间：
2015-03-12
影响因子：
5.6
通讯作者：
Hung CF
中科院分区：
文献类型：
--
作者：
Chan CM;Huang CH;Li HJ;Hsiao CY;Su CC;Lee PL;Hung CF
紫外线，尤其是UVA，可以穿透晶状体，到达视网膜，并对视网膜色素上皮（RPE）细胞产生氧化应激。尽管被蛋白质和DNA吸收较弱，但可能会引发活性氧（ROS）的产生并产生氧化损伤；视网膜色素上皮的氧化损伤被认为在年龄相关性黄斑变性（AMD）中发挥着重要作用。研究表明，白藜芦醇是红葡萄中丰富的活性成分，可以保护多种细胞类型免受氧化应激。本研究利用不同浓度的白藜芦醇处理成体RPE细胞，评估白藜芦醇对RPE细胞抵抗UVA诱导损伤的保护作用。细胞活力测定表明，白藜芦醇可减少 UVA 诱导的 RPE 细胞活力下降。通过流式细胞术分析，我们发现白藜芦醇能够以浓度依赖性方式抑制RPE细胞中UVA照射诱导的细胞内H2O2的产生。 Western blot分析结果表明，白藜芦醇降低了RPE细胞中UVA诱导的细胞外信号调节激酶、c-jun-NH2末端激酶和p38激酶的活化。此外，在用白藜芦醇预处理的 RPE 细胞中，UVA 诱导的环氧合酶 2 (COX-2) 表达也有所减少。我们的观察表明，白藜芦醇可有效防止 RPE 细胞受到 UVA 辐射的损伤，值得考虑进一步开发作为预防早期 AMD 的化学保护剂。
Ultraviolet radiation, especially UVA, can penetrate the lens, reach the retina, and induce oxidative stress to retinal pigment epithelial (RPE) cells. Even though it is weakly absorbed by protein and DNA, it may trigger the production of reactive oxygen species (ROS) and generate oxidative injury; oxidative injury to the retinal pigment epithelium has been implicated to play a contributory role in age-related macular degeneration (AMD). Studies showed that resveratrol, an abundant and active component of red grapes, can protect several cell types from oxidative stress. In this study, adult RPE cells being treated with different concentrations of resveratrol were used to evaluate the protective effect of resveratrol on RPE cells against UVA-induced damage. Cell viability assay showed that resveratrol reduced the UVA-induced decrease in RPE cell viability. Through flow cytometry analysis, we found that the generation of intracellular H2O2 induced by UVA irradiation in RPE cells could be suppressed by resveratrol in a concentration-dependent manner. Results of Western blot analysis demonstrated that resveratrol lowered the activation of UVA-induced extracellular signal-regulated kinase, c-jun-NH2 terminal kinase and p38 kinase in RPE cells. In addition, there was also a reduction in UVA-induced cyclooxygenase-2 (COX-2) expression in RPE cells pretreated with resveratrol. Our observations suggest that resveratrol is effective in preventing RPE cells from being damaged by UVA radiation, and is worth considering for further development as a chemoprotective agent for the prevention of early AMD.
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影响因子：
2.8
作者：
Brockmann, Claudia;Schulz, Marcus;Laube, Thomas
通讯作者：
Laube, Thomas
DOI：
10.1111/nyas.12196
发表时间：
2013-01-01
期刊：
RESVERATROL AND HEALTH
影响因子：
--
作者：
Ogas, Talysa;Kondratyuk, Tamara P.;Pezzuto, John M.
通讯作者：
Pezzuto, John M.
DOI：
10.1016/j.jphotobiol.2010.09.006
发表时间：
2011-01-10
影响因子：
5.4
作者：
Saitoh, Yasukazu;Miyanishi, Akifumi;Miwa, Nobuhiko
通讯作者：
Miwa, Nobuhiko
影响因子：
6.7
作者：
Mikulski, Damian;Gorniak, Rafal;Molski, Marcin
通讯作者：
Molski, Marcin
影响因子：
3.7
作者：
Chan C;Elliott J;Troughton R;Frampton C;Smyth D;Crozier I;Bridgman P
通讯作者：
Bridgman P