ERK knockdown suppress cell biological activities via regulation CD59 in breast cancer
ERK knockdown suppress cell biological activities via regulation CD59 in breast cancer
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ERK 敲低通过调节 CD59 抑制乳腺癌中的细胞生物活性
DOI：
10.5114/aoms/139683
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发表时间：
2021
影响因子：
3.8
通讯作者：
Qianwen Ouyang
中科院分区：
文献类型：
--
作者：
Fei Qu;Yanru Cui;Shixin Yang;Zhihua;Jingxian Ding;Wensong Wei;Yufeng Zou;Pinghua Hu;Haolong Ding;Zhibing Zhou;Qihua Jiang;Bing Zhou;Liping Yan;Qianwen Ouyang
ERK在乳腺癌发生发展中的作用及相关机制尚不清楚。本工作的目的是探讨ERK在乳腺癌中的作用及相关机制。通过IHC检测评估患者不同组织中ERK和CD59蛋白的表达。在我们的研究中，使用乳腺癌细胞系MCF-7和MDA-MB-231细胞系作为靶细胞系。体外研究，通过MTT、克隆试验、TUNEL实验、流式细胞术和伤口愈合评估细胞生物学活性，包括增殖、凋亡、细胞周期、侵袭、粘附和迁移。并通过WB实验测定相对蛋白表达。体内研究中，测量肿瘤重量和体积，TUNEL法检测凋亡细胞数，IHC法检测相对蛋白表达。与癌旁正常组织相比，乳腺癌组织中ERK和CD59蛋白表达显着升高（分别P＜0.001）。体外和体内研究，敲低ERK后，抑制CD59，细胞生物学活性显着降低（分别P＜0.001）。相关蛋白CD59、PKD、P53、E-cadherin和Vimentin差异显着(分别P＜0.001)。ERK在乳腺癌发生发展中发挥肿瘤基因作用，ERK抑制剂通过体内外研究通过调节CD59发挥抑制乳腺癌生物学作用。
It has been unclear that ERK play the effects and relative mechanism in breast cancer development. The purpose of this work was to discuss the ERK play the effect in breast cancer and relative mechanisms.Evaluating ERK and CD59 proteins expression in difference tissue from patients by IHC assay. Using MCF-7 and MDA-MB-231 cell lines which were breast cancer cell lines as target cell lines in our study. In vitro study, evaluating cell biological activities including proliferation, apoptosis, cell cycle, invasion, adherent and migration by MTT, clone test, TUNEL assay, flow cytometry and wound healing. And measuring relative proteins expressions by WB assay. In vivo study, measuring tumor weight and volume, the apoptosis cell number were evaluated by TUNEL assay and relative proteins expressions by IHC assay.Compared with adjacent normal tissue, the ERK and CD59 proteins expression were significantly increased in breast cancer tissues (P＜0.001, respectively).In vitro and vivo studies, with ERK knockdown, the cell biological activities were significantly depressed with CD59 suppressing (P＜0.001, respectively). And the relative proteins including CD59, PKD, P53, E-cadherin and Vimentin were significantly differences (P＜0.001, respectively).ERK play an oncology gene in breast cancer development, ERK inhibitor had effects to suppress breast cancer biological via regulation CD59 in vitro and vivo study.
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影响因子：
15.9
作者：
Bernhart E;Damm S;Heffeter P;Wintersperger A;Asslaber M;Frank S;Hammer A;Strohmaier H;DeVaney T;Mrfka M;Eder H;Windpassinger C;Ireson CR;Mischel PS;Berger W;Sattler W
通讯作者：
Sattler W
影响因子：
4.8
作者：
Ryvkin, Vladislav;Rashel, Mohammad;Ghazizadeh, Soosan
通讯作者：
Ghazizadeh, Soosan
影响因子：
--
作者：
Burnett RM;Craven KE;Krishnamurthy P;Goswami CP;Badve S;Crooks P;Mathews WP;Bhat-Nakshatri P;Nakshatri H
通讯作者：
Nakshatri H
肿瘤衍生补体的自分泌作用。
影响因子：
8.8
作者：
Cho MS;Vasquez HG;Rupaimoole R;Pradeep S;Wu S;Zand B;Han HD;Rodriguez-Aguayo C;Bottsford-Miller J;Huang J;Miyake T;Choi HJ;Dalton HJ;Ivan C;Baggerly K;Lopez-Berestein G;Sood AK;Afshar-Kharghan V
通讯作者：
Afshar-Kharghan V
影响因子：
3.5
作者：
E. Lim;N. Lin
通讯作者：
E. Lim;N. Lin