Characteristics of human CD34+ cells exposed to ionizing radiation under cytokine-free conditions.
Characteristics of human CD34+ cells exposed to ionizing radiation under cytokine-free conditions.
复制标题
DOI：
10.1093/jrr/rrv024
复制
发表时间：
2015-07
影响因子：
2
通讯作者：
Kashiwakura I
中科院分区：
文献类型：
--
作者：
Ishikawa J;Hayashi N;Yamaguchi M;Monzen S;Kashiwakura I
为了阐明辐射诱导造血干细胞死亡的机制，我们研究了在无细胞因子的条件下，过量电离辐射对人脐血CD34+细胞克隆形成能力的影响。CD34+细胞在无细胞因子的条件下进行0-48h的培养。在不同时间点检测CD34+细胞的存活率、克隆形成能力和线粒体超氧化物歧化能力。X射线照射后12h，活细胞数较未照射对照组下降至∼70~80%，而细胞克隆形成能力较未照射对照组下降至∼50%~60%。X射线照射后6h线粒体超氧化物歧化明显，12~24 h达最大值。然而，未照射和X射线照射的细胞在产生活性氧或细胞内线粒体含量方面没有显著差异。此外，基因芯片分析表明，X射线照射后6h，CD34+细胞中的变化基因大部分是与细胞凋亡相关的基因。这些结果提示，CD34+细胞克隆形成潜能的消除可能与电离辐射诱导线粒体超氧化物的产生有关。
To clarify the mechanisms underlying radiation-induced hematopoietic stem cell death, we investigated the effects of excessive ionizing radiation on the clonogenic potential of CD34+ cells obtained from human umbilical cord blood under cytokine-free conditions. The CD34+ cells were X-ray–irradiated (up to 2 Gy) and were cultured for 0–48 h under cytokine-free conditions. At various time-points, the CD34+ cells were investigated for survival, clonogenic potential and the generation of mitochondrial superoxide. At 12 h after X-ray irradiation, the number of viable cells had decreased to ∼70–80% compared with the 0-h non-irradiated control, whereas the clonogenic potential in the X-ray–irradiated cells had decreased to ∼50%–60% compared with the 0-h non-irradiated control. Furthermore, significant generation of mitochondrial superoxide was observed at 6 h, and reached a maximum value between 12 and 24 h after X-ray irradiation. However, no significant differences were observed between non-irradiated and X-ray–irradiated cells in terms of the generation of reactive oxygen species or in the intracellular mitochondrial contents. In addition, a cDNA microarray analysis showed that the majority of the altered genes in the CD34+ cells at 6 h after X-ray irradiation were apoptosis-related genes. These results suggest the possibility that the elimination of the clonogenic potentials of CD34+ cells involves the generation of mitochondrial superoxide induced by ionizing radiation.
登录
查看更多内容
影响因子：
3.4
作者：
Kato, Kengo;Takahashi, Kenji;Kashiwakura, Ikuo
通讯作者：
Kashiwakura, Ikuo
影响因子：
6.1
作者：
Hayashi, Naoki;Takahashi, Kenji;Kashiwakura, Ikuo
通讯作者：
Kashiwakura, Ikuo
影响因子：
8
作者：
Lee, S-H;Bahn, J. H.;Whitlock, N. C.;Baek, S. J.
通讯作者：
Baek, S. J.
影响因子：
5.2
作者：
Kim, H;Whartenby, KA;Civin, CI
通讯作者：
Civin, CI
影响因子：
3.4
作者：
Chu, K;Teele, N;Dewey, WC
通讯作者：
Dewey, WC