An Important Role of the Type VI Secretion System of Pseudomonas aeruginosa Regulated by Dnr in Response to Anaerobic Environments.
An Important Role of the Type VI Secretion System of Pseudomonas aeruginosa Regulated by Dnr in Response to Anaerobic Environments.
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Dnr调控的铜绿假单胞菌VI型分泌系统在厌氧环境反应中的重要作用
DOI：
10.1128/spectrum.01533-22
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发表时间：
2022-12-21
影响因子：
3.7
通讯作者：
Liang, Haihua
中科院分区：
文献类型：
--
作者：
Dang, Jing;Wang, Tietao;Wen, Jing;Liang, Haihua
VI型分泌系统（T6 SS）通过介导活性金属离子的转运，在细菌竞争中发挥重要作用。铜绿假单胞菌携带三个不同的T6 SS基因座（H1-、H2-和H3-T6 SS）。H2-T6 SS促进钼酸盐结合蛋白ModA的分泌，用于获得钼离子以适应厌氧生存。VI型分泌系统（T6 SS）能够分泌多种与金属离子摄取有关的金属结合蛋白，并介导一个活跃的金属离子转运系统，有助于细菌之间的竞争。铜绿假单胞菌H2-T6 SS可以通过促进钼酸盐结合蛋白ModA的分泌来增加钼离子获取并增强细菌存活优势，其中H2-T6 SS核心基因hcp 2、hsiA 2和clpV 2的表达由厌氧条件激活，并且都由全局调节因子Anr调节。在这里，我们报告的T6 SS的调节Dnr，一个专门的异化硝酸盐呼吸调节铜绿假单胞菌。在铜绿假单胞菌携带的三个不同的T6 SS位点中，只有H2-T6 SS的厌氧表达被Dnr激活; H1-T6 SS或H3-T6 SS对厌氧诱导的激活没有响应。我们还证明了Dnr促进ModA的厌氧分泌，ModA作为H2-T6 SS的潜在底物，不仅为细菌的厌氧生长提供了优势，而且还为功能竞争提供了优势。总之，本研究阐明了Dnr在介导铜绿假单胞菌中T6 SS厌氧表达中所起的重要作用，表明H2-T6 SS响应厌氧诱导的功能优势可能是一种条件性环境适应。这也扩展了我们对Dnr作为异化硝酸盐呼吸的特定调节剂的功能的理解。VI型分泌系统（T6 SS）通过介导活性金属离子的运输在细菌竞争中起着重要作用。铜绿假单胞菌携带三个不同的T6 SS基因座（H1-、H2-和H3-T6 SS）。H2-T6 SS促进钼酸盐结合蛋白ModA的分泌，用于获得钼离子以适应厌氧生存。在这里，我们报告说，专门异化硝酸盐呼吸调节剂Dnr在铜绿假单胞菌控制H2-T6 SS的厌氧表达，这种调节是必不可少的ModA蛋白分泌，厌氧生长和细菌竞争。本研究阐明了Dnr对铜绿假单胞菌H2-T6 SS的调控机制，揭示了H2-T6 SS在适应厌氧环境中的重要作用。
The type VI secretion system (T6SS) plays an important role in bacterial competition by mediating the transport of active metal ions. Pseudomonas aeruginosa carries three distinct T6SS loci (H1-, H2-, and H3-T6SS). The H2-T6SS promotes the secretion of the molybdate-binding protein ModA for the acquisition of molybdenum ions to adapt to anaerobic survival. ABSTRACT The type VI secretion system (T6SS) is capable of secreting a variety of metal-binding proteins involved in metal ion uptake, and it mediates an active metal ion transport system that contributes to competition between bacteria. Pseudomonas aeruginosa H2-T6SS can increase molybdenum ion acquisition and enhance bacterial survival advantage by promoting the secretion of the molybdate-binding protein ModA, in which the expression of H2-T6SS core genes hcp2, hsiA2, and clpV2 is activated by anaerobic conditions and are all regulated by the global regulator Anr. Here, we report the regulation of T6SS by Dnr, a dedicated dissimilatory nitrate respiration regulator in P. aeruginosa. Of the three distinct T6SS loci carried by P. aeruginosa, only the anaerobic expression of H2-T6SS was activated by Dnr; H1-T6SS or H3-T6SS did not respond to anaerobically induced activation. We also demonstrated that Dnr promotes the anaerobic secretion of ModA, which acts as a potential substrate for H2-T6SS, providing an advantage not only for the anaerobic growth of bacteria but also for functional competition. Overall, this study elucidates the important role played by Dnr in mediating the anaerobic expression of T6SS in P. aeruginosa, indicating that the functional advantage of H2-T6SS in response to anaerobic induction may be a conditional environmental adaptation. It also extends our understanding of the function of Dnr as a specific regulator of dissimilatory nitrate respiration. IMPORTANCE The type VI secretion system (T6SS) plays an important role in bacterial competition by mediating the transport of active metal ions. Pseudomonas aeruginosa carries three distinct T6SS loci (H1-, H2-, and H3-T6SS). The H2-T6SS promotes the secretion of the molybdate-binding protein ModA for the acquisition of molybdenum ions to adapt to anaerobic survival. Here, we report that the specialized dissimilatory nitrate respiration regulator Dnr in P. aeruginosa controls the anaerobic expression of H2-T6SS and that this regulation is essential for ModA protein secretion, anaerobic growth, and bacterial competition. This study elucidates the regulatory mechanism of Dnr on H2-T6SS in P. aeruginosa, revealing an important role played by H2-T6SS in adapting to an anaerobic environment.
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影响因子：
5.2
作者：
Arai H
通讯作者：
Arai H
影响因子：
3.2
作者：
Eschbach, M;Schreiber, K;Schobert, M
通讯作者：
Schobert, M
影响因子：
4.1
作者：
Kraft, Beate;Strous, Marc;Tegetmeyer, Halina E.
通讯作者：
Tegetmeyer, Halina E.
影响因子：
28.3
作者：
Burkinshaw, Brianne J.;Liang, Xiaoye;Dong, Tao G.
通讯作者：
Dong, Tao G.
DOI：
10.1098/rstb.2015.0021
发表时间：
2015-10-05
期刊：
Philosophical transactions of the Royal Society of London. Series B, Biological sciences
影响因子：
--
作者：
Basler M
通讯作者：
Basler M