Molecular characterization of a novel recombinant strain of human astrovirus associated with gastroenteritis in children.
Molecular characterization of a novel recombinant strain of human astrovirus associated with gastroenteritis in children.
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DOI：
10.1007/s007050170008
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发表时间：
2001-12
影响因子：
2.7
通讯作者：
Mitchell DK
中科院分区：
文献类型：
--
作者：
Walter JE;Briggs J;Guerrero ML;Matson DO;Pickering LK;Ruiz-Palacios G;Berke T;Mitchell DK
我们报告了在两个不同地理位置检测到的自然存在的人类星状病毒（HAstV）毒株。我们在德克萨斯州休斯敦一家儿童保育中心的腹泻爆发中发现了该菌株的两种分离株；以及墨西哥城两名儿童腹泻粪便样本中的两种分离株。通过酶免疫分析 (EIA) 在粪便样本中检测到所有四种分离株。其中一个墨西哥分离株通过 EIA 进行分型，所有四个分离株通过 RT-PCR 分型均为 HAstV-5。四个分离株在两个不同的基因组区域中的核苷酸同一性 >97%：ORF1a (246nt) 和基因组 3' 末端 (471nt)。来自每个地理位置的一个分离株在从 ORF1b 到 ORF2 (1255nt) 的过渡区域中被进一步测序，并且两个分离株的该区域显示出 ≥99%nt 同一性。对八种HAstV抗原类型和过渡区新毒株序列的系统发育分析表明，新毒株与ORF1b中的HAstV-3密切相关，但与ORF2中的HAstV-5最接近。这些结果以及过渡区所有 HAstV 抗原类型之间的高序列同一性和 RNA 结构预测支持 ORF1b/ORF2 连接处存在潜在的重组位点。这是人类星状病毒之间发生重组的第一个证据。
We report a naturally occurring human astrovirus (HAstV) strain detected in two different geographic locations. We identified two isolates of this strain in a diarrhea outbreak at a child care center in Houston, Texas; and two isolates in diarrhea stool samples from two children in Mexico City. All four isolates were detected in stool samples by enzyme immunoassay (EIA). One of the Mexican isolates was typed by EIA and all four isolates were HAstV-5 by typing RT-PCR. The four isolates were >97% nucleotide-identical in two different genomic regions: ORF1a (246nt), and the 3′ end of the genome (471nt). One isolate from each geographic location was further sequenced in the transition region from ORF1b to ORF2 (1255nt) and this region of the two isolates showed ≥ 99% nt identity. Phylogenetic analyses of sequences of eight HAstV antigenic types and the novel strain in the transition region demonstrated the new strain being closely related to HAstV-3 in ORF1b, but closest to HAstV-5 in ORF2. These results and high sequence identity among all HAstV antigenic types in the transition region and RNA structural predictions supported a potential recombination site at the ORF1b/ORF2 junction. This is the first evidence that recombination occurs among human astroviruses.
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影响因子：
2.7
作者：
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通讯作者：
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