Selective capture of transcribed sequences in the functional gene analysis of microbial pathogens
Selective capture of transcribed sequences in the functional gene analysis of microbial pathogens
复制标题
微生物病原体功能基因分析中转录序列的选择性捕获
DOI:
10.1007/s00253-014-6190-9
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发表时间:
2014-11
影响因子:
5
通讯作者:
Ding, Chan
中科院分区:
文献类型:
--
作者:
Yi, Li;Wang, Shaohui;Lu, Chengping;Ding, Chan
Selective capture of transcribed sequences (SCOTS) is an effective method to identify bacterial genes differentially expressed during different biological processes, including pathogenic interactions with a host species. The method can be used to elucidate molecular mechanisms driving and maintaining such interactions. The method is a powerful genetic tool that overcomes limitations found in other methods, by working with small amounts of mRNA and allowing for the separation of bacterial cDNA from host cDNA. It has been increasingly used in the discovery of genes involved in the bacterium-host interaction. In this review, we briefly introduce the SCOTS method, outline the technical advances offered in the method, and focus on the method’s applications in several microbial pathogens.
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影响因子:
3.7
作者:
Guo CM;Chen RR;Kalhoro DH;Wang ZF;Liu GJ;Lu CP;Liu YJ
通讯作者:
Liu YJ
影响因子:
14.9
作者:
Chen LY;Wei KC;Huang AC;Wang K;Huang CY;Yi D;Tang CY;Galas DJ;Hood LE
通讯作者:
Hood LE
DOI:
--
发表时间:
2013
期刊:
--
影响因子:
--
作者:
Samuel Myllykangas;Juha W. Koskenvuo;Tero-Pekka Alastalo
通讯作者:
Samuel Myllykangas;Juha W. Koskenvuo;Tero-Pekka Alastalo
影响因子:
3.3
作者:
Baltes, Nina;Buettner, Falk F. R.;Gerlach, Gerald-F.
通讯作者:
Gerlach, Gerald-F.
影响因子:
3
作者:
C. Winstanley
通讯作者:
C. Winstanley