Identification of genes preferentially expressed by highly virulent piscine Streptococcus agalactiae upon interaction with macrophages.
Identification of genes preferentially expressed by highly virulent piscine Streptococcus agalactiae upon interaction with macrophages.
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DOI：
10.1371/journal.pone.0087980
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发表时间：
2014
期刊：
影响因子：
3.7
通讯作者：
Liu YJ
中科院分区：
文献类型：
--
作者：
Guo CM;Chen RR;Kalhoro DH;Wang ZF;Liu GJ;Lu CP;Liu YJ
无乳链球菌，长期以来被认为是一种哺乳动物病原体，是一个新兴的关注方面的鱼类。本研究采用小鼠模型和体外细胞感染的方法，研究了沙门氏菌的致病特性。无乳杆菌GD 201008 -001，分离自中国罗非鱼。这种细菌被发现是高毒性的，并且能够通过穿过血脑屏障（BBB）迁移到大脑中来诱导脑损伤。吞噬试验表明，该细菌可被小鼠巨噬细胞内化，并在细胞内存活超过24小时，诱导巨噬细胞损伤。此外，转录序列的选择性捕获（SCOTS）被用来研究与细胞内存活相关的微生物基因表达。这种积极的cDNA选择技术确定了60个不同的基因，可以分为6个功能类别。超过50%的差异表达基因与代谢适应有关。一些基因先前被描述为与其他细菌的毒力相关，其中四个基因与任何其他先前描述的基因没有显着相似性。这项研究构成了进一步基因表达分析的第一步，这将导致更好地理解S。在巨噬细胞中存活并穿过血脑屏障。
Streptococcus agalactiae, long recognized as a mammalian pathogen, is an emerging concern with regard to fish. In this study, we used a mouse model and in vitro cell infection to evaluate the pathogenetic characteristics of S. agalactiae GD201008-001, isolated from tilapia in China. This bacterium was found to be highly virulent and capable of inducing brain damage by migrating into the brain by crossing the blood–brain barrier (BBB). The phagocytosis assays indicated that this bacterium could be internalized by murine macrophages and survive intracellularly for more than 24 h, inducing injury to macrophages. Further, selective capture of transcribed sequences (SCOTS) was used to investigate microbial gene expression associated with intracellular survival. This positive cDNA selection technique identified 60 distinct genes that could be characterized into 6 functional categories. More than 50% of the differentially expressed genes were involved in metabolic adaptation. Some genes have previously been described as associated with virulence in other bacteria, and four showed no significant similarities to any other previously described genes. This study constitutes the first step in further gene expression analyses that will lead to a better understanding of the molecular mechanisms used by S. agalactiae to survive in macrophages and to cross the BBB.
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