The application of 8-aminoguanosine triphosphate, a new inhibitor of GTP cyclohydrolase I, to the purification of the enzyme from human liver.
The application of 8-aminoguanosine triphosphate, a new inhibitor of GTP cyclohydrolase I, to the purification of the enzyme from human liver.
复制标题
新型GTP环化水解酶I抑制剂8-氨基鸟苷三磷酸在纯化人肝脏酶中的应用。
DOI：
10.1016/0304-4165(86)90115-7
复制
发表时间：
1986
期刊：
影响因子：
--
通讯作者：
A. Niederwieser
中科院分区：
文献类型：
--
作者：
N. Blau;A. Niederwieser
GTP cyclohydrolase I from human liver andEscherichia coliis competitively inhibited by 8-aminoguanosine triphosphate with a dissociation constant (Ki) of 0.25 μmol/1. 8-Aminoguanosine triphosphate, prepared from GTP and hydroxylamine-O-sulfonic acid, was coupled to Sepharose 4B and used as an affinity adsorbent for a 309-fold purification of GTP cyclohydrolase I from human liver. GTP cyclohydrolase I from human liver is a relatively heat-stable enzyme with a half-life of 2 min at 80°C, an isoelectric point (pI) of about 5.6, and aKmfor GTP of 31 μmol/I. Addition of KCl (0.3 mol/I) increased theKmto 153 μmol/I. No cofactors were required for activity.L-erythro-5,6,7,8-Tetrahydrobiopterin,L-erythro-7,8-dihydrobiopterin,L-sepiapterin and 8-aminoguanosine triphosphate were strong inhibitors.
8-氨基鸟苷抑制嘌呤核苷磷酸化酶：对 T 淋巴细胞的选择性毒性。
DOI：
10.1126/science.6795718
发表时间：
1981
期刊：
Science (New York, N.Y.)
影响因子：
--
作者：
Kazmers,IS;Mitchell,BS;Dadonna,PE;Wotring,LL;Townsend,LB;Kelley,WN
通讯作者：
Kelley,WN